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作 者:吉莉[1] 李娜 王新明 钱天伟[1] 张桂香[1] 胡变芳[3] JI Li;LI Na;WANG Xinming;QIAN Tianwei;ZHANG Guixiang;HU Bianfang(College of Environment and Safety,Taiyuan University of Science and Technology,Taiyuan 030024,China;Guangdong Provincial Key Laboratory of Environmental Protection and Resources Utilization,Guangzhou 510640,China;Department of Biology,Jinzhong University,Jinzhong 030600,China)
机构地区:[1]太原科技大学环境与安全学院,山西太原030024 [2]广东省环境资源利用与保护重点实验室,广东广州510640 [3]晋中学院生物科学与技术学院,山西晋中030600
出 处:《山西大学学报(自然科学版)》2021年第3期617-624,共8页Journal of Shanxi University(Natural Science Edition)
基 金:山西省留学回国人员科技活动择优项目(2018);广东省省级科技计划项目(2017B030314057);山西省高等学校科技创新项目2019L0613。
摘 要:锝(^(99)Tc)是一种放射性核素,对人类健康具有潜在威胁。硫酸盐还原菌(sulfate-reducing bacteria,SRB)可以与重金属反应生成稳定的金属硫化物沉淀,防止其迁移扩散。由于锝的样品难于获得,文章用铼作为锝的替代元素,采用单因素实验和L_(9)(3^(4))正交实验,研究了初始p H、培养温度、初始铼酸根(ReO_(4)^(-))浓度对SRB还原ReO_(4)^(-)效果的影响。结果表明,单因素条件下,当p H为7.5、培养温度为35℃、ReO_(4)^(-)初始浓度为10μg/m L时,SRB对ReO_(4)^(-)的降解效率最佳,降解率为63.01%。正交实验条件下,当p H为7.5、培养温度为35℃、ReO_(4)^(-)初始浓度为5μg/m L时,SRB对ReO_(4)^(-)的降解效率最佳,降解率为64.21%。ReO_(4)^(-)的初始浓度是SRB降解ReO_(4)^(-)的主要因素,其次是温度和pH。Technetium (^(99)Tc) is a radionuclide,which is a potential threat to human health.Sulfate-reducing bacteria (SRB) could react with heavy metals to form stable metal sulfide precipitates,preventing their migration and diffusion.Due to the difficulty in obtaining the sample of Fe,the Re was employed as its substitute in this work.Single-factor experiments and L_(9)(3^(4)) orthogonal experiments were used to study the effects of initial p H,culture temperature,and initial concentration on ReO_(4)^(-)removal by the SRB.The results showed that under single factor conditions,the best degradation condition was the initial p H value of 7.5,the culture temperature at 35℃,and the initial ReO_(4)^(-)concentration of 10μg/m L,with a degradation rate of 63.01%.Under orthogonal experimental conditions,the best degradation condition was the initial pH 7.5,the culture temperature at 35℃,and the initial ReO_(4)^(-)concentration of 5μg/m L,with a degradation rate of 64.21%.The initial concentration of ReO_(4)^(-)was the key factor influencing the removal efficiency,followed by temperature and pH.
关 键 词:硫酸盐还原菌 ReO_(4)^(-) 正交实验 条件优化
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