硼处理根瘤菌接种液对紫花苜蓿根、茎、叶中可溶性糖含量的影响  

Effects of boron-treated rhizobial inoculum on soluble sugar concentration in alfalfa roots,stems,and leaves

作　　者：陈永岗 师尚礼[1] 吴芳 康文娟 张辉辉 阿芸 李兴龙 陈建刚[1] CHEN Yonggang;SHI Shangli;WU Fang;KANG Wenjuan;ZHANG Huihui;A Yun;LI Xinglong;CHEN Jian'gang(College of Grassland Science,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃农业大学草业学院,甘肃兰州730070

出　　处：《草业科学》2021年第6期1138-1146,共9页Pratacultural Science

基　　金：国家农业产业技术体系项目(CARS-34)。

摘　　要：以甘农5号紫花苜蓿(Medicago sativa‘Gannong No.5’)为材料,设置未添加硼且未接菌的紫花苜蓿为对照和添加不同硼浓度(0、0.05、1、5、10和100 mg·L^(-1))的两种根瘤菌菌液(Sinorhizobium meliloti LZgn5f和S.meliloti 12531f)接种紫花苜蓿幼苗根部,研究硼处理根瘤菌接种对不同时期根、茎、叶中可溶性糖含量的影响。结果表明:1 mg·L^(-1)硼处理外源根瘤菌12531f和100 mg·L^(-1)硼处理内源根瘤菌gn5f接种后,不同取样时期苜蓿根和茎中可溶性糖含量均高出对照和单独接菌处理,且可以促进不同时期苜蓿体内可溶性糖向根和茎中运输积累。综上所述,适宜的硼处理两菌株均可促进不同生长时期叶中可溶性糖向根和茎中运输积累,保持根、茎、叶中可溶性糖的动态平衡。We aimed to explore the effects of boron-treated rhizobial inocula on the soluble sugar content in the roots,stems,and leaves in different periods.Medicago sativa‘Gannong No.5’alfalfa was used as the test material;two rhizobial solutions(from Sinorhizobium meliloti LZgn5f and S.meliloti 12531 f)containing different boron concentrations(0,0.05,1.5,10,and100 mg·L^(-1))were inoculated into alfalfa seedling roots,and no boron and inoculum were used as the control.The results showed that after inoculation with exogenous rhizobia 12531 f containing 1 mg·L^(-1) boron and endogenous rhizobia gn5 f containing 100 mg·L^(-1) boron,the soluble sugar content in alfalfa roots and stems at different sampling periods was higher than that in both the control and no-boron inoculation treatments and could promote the accumulation of soluble sugars in the roots and stems of alfalfa in different periods.In summary,under suitable boron treatment concentrations,the two strains could promote the accumulation of soluble sugars in the leaves to roots and stems in different growth periods and maintain the dynamic balance of soluble sugars in the roots,stems,and leaves.

关 键 词：硼 紫花苜蓿 荧光标记根瘤菌 可溶性糖 动态平衡 接种 

分 类 号：S541.9[农业科学—作物学]