重金属溶液中微藻物质光谱变化的研究  

Spectral changes of microalgae in heavy metal solution

作　　者：熊鑫 王雨田 邵咏妮 XIONG Xin;WANG Yutian;SHAO Yongni(School of Optical-Electrical and Computer Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China)

机构地区：[1]上海理工大学光电信息与计算机工程学院,上海200093

出　　处：《光学仪器》2021年第3期60-64,共5页Optical Instruments

基　　金：上海市自然科学基金(18ZR1425700)。

摘　　要：针对传统水体重金属检测方法存在样本预处理耗时、成本高等问题,提出了一种基于太赫兹、远红外光谱技术对物质的指纹谱识别能力的斜生栅藻的光谱检测方法。根据微藻在吸附水体重金属后其主要成分含量会发生变化的特性,采用傅里叶红外太赫兹光谱仪采集了微藻在重金属Pb2+浓度分别为0 mg/L、0.005 mg/L、0.010 mg/L、0.020 mg/L、0.050 mg/L的溶液中,吸附10 min后,在1~20 THz范围内的光谱信号。利用藻样在太赫兹和远红外范围内特征峰处的吸光度值判断水体的污染程度。通过偏最小二乘(PLS)研究了不同质量浓度重金属溶液下的藻体样本是否能被区分开。实验结果表明,不同重金属质量浓度下的微藻存在差异,为水体重金属质量浓度的实时监测提供了参考。Traditional water heavy metal detection methods include atomic absorption spectrophotometer,inductive coupling method,etc.,but there are problems such as time-consuming sample pretreatment and high cost.Based on the fingerprint identification ability of terahertz and far infrared spectroscopy technology,the concentration of heavy metals is determined by studying the changes of the main substances after the adsorption of heavy metals in the contaminated water.The experiment takes the heavy metals Pb2+as the objects,and uses Fourier infrared spectrometer to collect the Spectral signals of scenedesmus obliquus samples in the heavy metals Pb2+(0 mg/L,0.005 mg/L,0.010 mg/L,0.020 mg/L,and 0.050 mg/L)in the range of 1~20 THz.Using the absorbance values of algae samples at characteristic peaks in the terahertz and far-infrared range,changes in the composition of substances in the body are studied.Partial least squares(PLS)is used to determine whether the algae samples in different concentrations of heavy metal solutions can be distinguished. The experimental results show that there are differences in the microalgae under different concentrations of heavy metals, which provides a reference for the real-time monitoring of the concentrations of heavy metals in water.

关 键 词：太赫兹 偏最小二乘(PLS) 斜生栅藻 铅 

分 类 号：O433.4[机械工程—光学工程]