美丽崖豆藤炭疽病病原鉴定及防治药剂的室内毒力测定  

Pathogen identification of anthracnose disease on Millettia speciosa and indoor determination of fungicide toxicity

作　　者：徐丹丹 石力允[2] 林泽勉 李依庭 姜子德 乔方[1] XU Dandan;SHI Liyun;LIN Zemian;LI Yiting;JIANG Zide;QIAO Fang(Department of Applied Chemistry and Biotechnology/Postdoctoral Innovation Practice Base,Shenzhen Polytechnic,Shenzhen 518055,China;College of Plant Protection,South China Agricultural University/Guangdong Provincial Key Laboratory of Microbial Signals and Disease Control,Guangzhou 510642,China)

机构地区：[1]深圳职业技术学院应用化学与生物技术学院/深圳职业技术学院博士后创新实践基地,广东深圳518055 [2]华南农业大学植物保护学院/广东省微生物信号与作物病害防控重点实验室,广东广州510642

出　　处：《华南农业大学学报》2021年第4期63-69,共7页Journal of South China Agricultural University

基　　金：国家现代农业产业体系(CARS-32);深圳职业技术学院博士后启动基金(6019330008K)。

摘　　要：【目的】明确引起美丽崖豆藤炭疽病的病原菌种类并筛选其防治药剂。【方法】采用组织分离法对病原菌进行分离、纯化后,利用柯赫氏法则验证其致病性,依据菌株的形态学特征和多基因序列分析确定病原菌种类;采用菌丝生长速率法测定病原菌对生产上常用于炭疽病防治的4种杀菌剂的敏感性。【结果】分离得到的6株菌中有2株菌可侵染美丽崖豆藤叶片引起褐色病斑;结合形态学鉴定和多基因序列分析,确定引起美丽崖豆藤炭疽病的病原菌为暹罗刺盘孢Colletotrichum siamense。该病菌对苯醚甲环唑、咪鲜胺、吡唑醚菌酯和甲基硫菌灵的敏感性均高,抑制中浓度(EC50)均小于0.1 mg/L,其中,以咪鲜胺的防效最佳,EC50为0.015 mg/L。【结论】美丽崖豆藤炭疽病的病原菌为暹罗刺盘孢,苯醚甲环唑、咪鲜胺、吡唑醚菌酯和甲基硫菌灵可作为防治美丽崖豆藤炭疽病的首选药剂。【Objective】To determine the pathogen causing anthracnose disease on Millettia speciosa and screen effective fungicides.【Method】Tissue isolation method was used to isolate the pathogen.The pathogenicity was tested according to Koch’s rule after purification.The pathogen was identified based on morphological characteristics and multi-locus sequence analysis.Furthermore,the sensitivity of this pathogen against four common fungicides was measured according to the mycelial growth rate.【Result】Two out of six obtained isolates could infect M.speciosa leaf and caused brown spot.Combining morphological characteristics and multi-locus sequences analysis,the pathogen caused anthracnose disease on M.speciosa was identified as Colletotrichum siamense.This pathogen was highly sensitive to difenoconazole,prochloraz,pyraclostrobin and thiophanate-methyl with EC50 values below 0.1 mg/L,and prochloraz showed the highest efficacy with EC50 of 0.015 mg/L.【Conclusion】The pathogen causing anthracnose disease on M.speciosa is C.siamense,and difenoconazole,prochloraz,pyraclostrobin and thiophanate-methyl can be applied to control anthracnose disease in the field.

关 键 词：美丽崖豆藤 炭疽病 暹罗刺盘孢 形态特征 多基因序列分析 防治药剂 

分 类 号：R282.2[医药卫生—中药学] S432.4[医药卫生—中医学]