Monoclonal antibody based Dot-ELISA and indirect fluorescence antibody technique for detecting Edwardsiella ictaluri infection in yellow catfish (Pelteobagrus fulvidraco)  

作　　者：Ruijun Li Hua Huang Xianyu Zhang Shigen Ye Qiang Li 

机构地区：[1]Dalian Key Laboratory of Marine Animal Disease Control and Prevention,Agriculture Department Key Laboratory of Mariculture&Stock Enhancement in North China's Sea,Dalian Ocean University,Dalian 116023,China [2]Department of Ocean Technology,College of Marine and Biology Engineering,Yancheng Institute of Technology,Yancheng 224051,China

出　　处：《Aquaculture and Fisheries》2017年第5期207-212,共6页渔业学报（英文）

基　　金：This study was supported by Public Science and Technology Research Funds Projects of Ocean(201405003);Cultivation Plan for Youth Agricultural Science and Technology Innovative Talents of Liaoning Province(2014003);Program of Liaoning Excellent Talents in University(LJQ2011075)to Prof.Qiang Li,General Scientific Research Project of Liaoning Education Department(L2015077);Doctoral Research Program of Liaoning Science and Technology Department(201601287)to Dr.Ruijun Li.

摘　　要：Edwardsiella ictaluri is known as the etiological agent of Red-head disease of yellow catfish(Pelteobagrus fulvidraco),and is the cause of heavy economic losses in the aquaculture industry.In this study,a DotEnzyme Linked Immunosorbent Assay(Dot-ELISA)and an Indirect Fluorescence Antibody technique(IFAT)for detecting Edwardsiella ictaluri were developed by using a monoclonal antibody,5D11.For DotELISA,the working dilutions of 5D11 and the secondary antisera(enzyme-labeled goat anti-mouse Ig)were 1:320 dilution and 1:3000 dilution,respectively.For IFAT,the working dilutions of 5D11 and goatanti-mouse Ig conjugated with fluorescein isothiocyanate were as respectively 1:80 and 1:256.Both of the methods established were highly sensitivity(Minimum detectable concentration,5
107 CFU/mL)and had a high degree of accuracy(positive rate was 100%for both artificial infected fish and spontaneous diseased Pelteobagrus fulvidraco).The two reliable methods developed have high potential for quick and efficient detection of Edwardsiella ictaluri in aquaculture production units.

关 键 词：Edwardsiella ictaluri Monoclonal antibody DOT-ELISA IFAT 

分 类 号：S85[农业科学—兽医学]