Ag2S@BSA存储液对纯钛表面成骨细胞行为的影响  

Effects of a AgS@BSA-containing storage solution on osteoblasts behaviors on pure titanium surface

作　　者：汤泽华 朱文卿 邱憬[1] TANG Zehua;ZHU Wenqing;QIU Jing(Department of Oral Implantology,the Affiliated Stomatological Hospital of Nanjing Medical University,Jiangsu Province Key Laboratory of Oral Diseases,Jiangsu Privince Engineering Research Center of Stomatological Trarslational Medicine,Nanjing 210029,China)

机构地区：[1]南京医科大学附属口腔医院种植科,江苏省口腔疾病研究重点实验室,江苏省口腔转化医学工程研究中心,江苏南京210029

出　　处：《南京医科大学学报（自然科学版）》2021年第5期670-676,共7页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金面上项目(81870799);江苏省重点研发计划(社会发展)项目(BE2019728);江苏省青年医学人才项目(QNRC2016850);南京医科大学-苏盐智创新研发基金项目(NMU-SY201806);镇江市重点研发计划(社会发展)项目(SH2019040);江苏高校优势学科建设工程资助项目(2018-87)。

摘　　要：目的:研究含牛血清白蛋白(bovine serum albumin,BSA)包裹的硫化银存储液对纯钛表面特性及成骨细胞生物学行为的影响。方法:纯钛试件经打磨清洗后分为4组,分别置于空气、生理盐水、BSA溶液、牛血清白蛋白包裹的硫化银溶液(BSAcoated Ag2S solution,Ag2S@BSA)中保存2周后,采用扫描电镜、X线能谱仪和光学接触角仪分析纯钛表面特性的变化;将MC3T3-E1成骨细胞接种于各组钛片表面,观察成骨细胞的黏附、增殖和分化行为。结果:扫描电镜和能谱分析结果显示,各组钛表面微形貌无明显差异,空气存储后未见明显改变,经生理盐水存储后钛表面散在分布氯化钠晶体,经BSA溶液存储后含碳有机物增加,经Ag2S@BSA存储后钛表面均匀吸附含硫和银元素的纳米颗粒。与其余3种存储方式相比,Ag2S@BSA溶液存储可显著减小钛表面水接触角,增大表面能,显著促进成骨细胞的黏附、增殖和成骨功能蛋白表达。结论:Ag2S@BSA存储液能优化钛表面特性,增强其成骨活性。Objective:This study aims to investigate the effects of bovine serum albumin(BSA)coated Ag2S storage solution on the surface characteristics of pure titanium and the behaviors of osteoblasts.Methods:After polishing and cleaning,pure titanium disks were stored in air,saline,BSA solution and BSA-coated Ag2S storage solution(Ag2S@BSA)respectively for two weeks.Scanning electron microscope(SEM),energy dispersive spectrometer(EDS)and contact angle test were used to analyze surface characteristics of pure titanium preserved in four media.Assays of adhesion,proliferation,and differentiation of MC3T3-E1 cells were conducted to evaluate the biocompatibility of different samples.Results:SEM and EDS results demonstrated that sodium chloride crystals were scattered on titanium surface in the saline group,while carbon and oxygen distribution were denser in the BSA group.Nano-sized particles containing silver and sulfur were adsorbed on the titanium surface without changing the original morphology.Compared with the other three groups,titanium surfaces preserved in Ag2S@BSA solution showed superior hydrophilicity and significantly promoted the adhesion proliferation and differentiation abilities of osteoblasts with high expression of osteogenesis-related proteins.Conclusion:The Ag2S@BSA-containing storage solution can enhance the osteogenic activity of osteoblasts by improving the surface characteristics of pure titanium.

关 键 词：牛血清白蛋白 AG2S 存储液 钛 成骨细胞 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]