基于TGF-β1/Smad3信号通路探讨芪棱汤对急性脑梗死大鼠神经功能的保护作用  

Discussion on Protective Effect of Qileng Tang on Neurological Function in Rats with Acute Cerebral Infarction Based on TGF-β1/Smad3 Signaling Pathway

作　　者：潘涛 潘东波 陈卫东 倪秀渊 PAN Tao;PAN Dongbo;CHEN Weidong;NI Xiuyuan

机构地区：[1]温州老年病医院神经内科,浙江温州325000

出　　处：《新中医》2021年第12期1-6,共6页New Chinese Medicine

摘　　要：目的:探讨芪棱汤对急性脑梗死大鼠神经功能的保护作用及其作用机制。方法:将120只SD大鼠随机分成对照组、模型组、丁苯酞组及芪棱汤低、中、高剂量组,每组20只,雌雄各半。除对照组外,其余各组建立大鼠急性脑梗死模型,模型制备成功后,丁苯酞组、芪棱汤各剂量组给予相应的药物灌胃,对照组、模型组给予等量生理盐水,持续7 d。实验结束后,对大鼠进行神经功能评分,并获得双侧贴纸去除时间、平衡木过杆评分,测定大鼠脑转化生长因子-β1(TGF-β1)、Smad3蛋白及mRNA相对表达,白细胞介素-2(IL-2)、白细胞介素-4(IL-4)、肿瘤坏死因子-α(TNF-α)蛋白表达。结果:与对照组比较,模型组神经运动功能评分降低(P<0.05),双侧贴纸去除时间,平衡木过杆评分,IL-2、IL-4、TNF-α蛋白表达水平,TGF-β1、Smad3蛋白与mRNA相对表达水平均升高(P<0.05)。与模型组比较,丁苯酞组、芪棱汤各剂量组神经运动功能评分升高(P<0.05),双侧贴纸去除时间,平衡木过杆评分,IL-2、IL-4、TNF-α蛋白表达水平,TGF-β1、Smad3蛋白与mRNA相对表达水平均降低(P<0.05),且随着芪棱汤剂量的增加,神经运动功能评分逐渐升高(P<0.05),双侧贴纸去除时间,平衡木过杆评分,IL-2、IL-4、TNF-α蛋白表达水平,TGF-β1、Smad3蛋白与mRNA相对表达水平逐渐降低(P<0.05)。与丁苯酞组比较,芪棱汤低、中剂量组神经运动功能评分降低(P<0.05),双侧贴纸去除时间,平衡木过杆评分,IL-2、IL-4、TNF-α蛋白表达水平,TGF-β1、Smad3蛋白与mRNA相对表达水平均升高(P<0.05)。结论:芪棱汤对急性脑梗死大鼠神经功能损伤有保护作用,其机制与芪棱汤通过抑制TGF-β1、Smad3 mRNA和蛋白表达进而抑制IL-2、IL-4、TNF-α炎症因子的产生有关。Objective:To discuss the protective effect of Qileng tang on neurological function in rats with acute cerebral infarction and its mechanism.Methods:A total of 120 SD rats were randomly divided into the control group,the model group,the butylphthalide group,and the Qileng tang groups with low dose,medium dose,and high dose,20 rats in each group,half male and half female.Except for the control group,rat models with acute cerebral infarction were established in the other groups.After successful modeling,the Qileng tang groups with low dose,medium dose,and high dose as well as the butylphthalide group were given gastric perfusion with the corresponding medicines;the control group and the model group were given the same volume of normal saline;the interventions above lasted for seven days.After experiment,neurological function of rats was scored;the removal time of bilateral stickers and the score of balance beam walking were counted;the relative expression of protein and mRNA of transforming growth factor-β1(TGF-β1)and Smad3 in the brain of rats were determined;the expression of protein of interleukin-2(IL-2),interleukin-4(IL-4),and tumor necrosis factor-α(TNF-α)was determined.Results:Compared with those in the control group,in the model group,the score of neuromotor function was decreased(P<0.05);the removal time of bilateral stickers,the score of balance beam walking,the expression levels of protein of IL-2,IL-4,and TNF-α,as well as the relative expression levels of protein and mRNA of TGF-β1 and Smad3 were increased(P<0.05).Compared with those in the model group,in the butylphthalide group and the Qileng tang groups with low dose,medium dose,and high dose,scores of neuromotor function were increased(P<0.05);the removal time of bilateral stickers,scores of balance beam walking,the expression levels of protein of IL-2,IL-4,and TNF-α,as well as the relative expression levels of protein and mRNA of TGF-β1 and Smad3 were decreased(P<0.05).Compared with those in the model group,in the Qileng tang groups with low

关 键 词：急性脑梗死 芪棱汤 转化生长因子-Β1 SMAD3 神经功能 动物实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]