基于全基因组序列分析小西葫芦黄花叶病毒的基因组特征和进化关系  

Genome Characteristics and Evolution of Zucchini Yellow Mosaic Virus Based on the Shanxi Isolate of Cucurbita pepo L.in China

作　　者：王德富 崔丽艳 王舒婷 王疆然 郭尚 牛颜冰 WANG De-Fu;CUI Li-Yan;WANG Shu-Ting;WANG Jiang-Ran;GUO Shang;NIU Yan-Bing(Department of Traditional Chinese Medicine,College of Life Sciences,Shanxi Agricultural University,Taigu 030801,Shanxi,China;Department of Edible Fungi,Shanxi Institute for Functional Food,Shanxi Agricultural University,Taiyuan 030006,China)

机构地区：[1]山西农业大学生命科学学院中药科学系,山西太谷030801 [2]山西农业大学山西功能食品研究院食用菌研究室,太原030006

出　　处：《中国生物化学与分子生物学报》2021年第6期811-821,共11页Chinese Journal of Biochemistry and Molecular Biology

基　　金：国家自然科学基金项目(No.31772130);财政部和农业农村部:国家现代农业产业技术体系(No.CARS-21)资助。

摘　　要：小西葫芦黄花叶病毒(zucchini yellow mosaic virus,ZYMV)作为葫芦科作物的主要为害病毒之一,严重影响其产量和品质。目前,有关ZYMV遗传进化研究以CP序列为主,而针对全基因组水平系统进化关系研究却少见报道。有鉴于此,本研究以ZYMV山西分离物(ZYMV-WS)和已报道的其他109个ZYMV分离物的全基因组序列为对象,探索其系统进化关系。结果表明,ZYMVWS分离物的核苷酸与氨基酸分别与ZYMV-SXSG(94.35%)和ZYMV-BR3(97.37%)相似性最高;系统进化分析表明,ZYMV-WS与已报道的109个ZYMV分离物主要分为A和B 2个亚组,种群结构分析表明,A4和B群体之间的遗传变异最大;氨基酸突变与功能分析发现,ZYMV存在与蛋白质功能相关的氨基酸突变。上述结果有助于全面了解ZYMV的遗传多样性和系统进化进程,为今后积极开展ZYMV的有效防控奠定了理论基础。Zucchini yellow mosaic virus(ZYMV)is a destructive cucurbit virus that causes extensive losses in the yield and quality of cucurbits(e.g.,zucchini).At present,the analysis of genetic evolution of ZYMV is mainly based on CP sequence,and there has been little progress in addressing its phylogenetic relationships and evolution at the whole genome level.In this study,the whole genome sequences of ZYMV isolated from Shanxi Wenshui(ZYMV-WS)and 109 other reported ZYMV isolates were used to explore their phylogenetic relationships.The results showed that ZYMV-WS had the highest level of nucleotide homology(94.35%)to ZYMV-SXSG,and the amino acid sequences of ZYMV-BR3 were identical(97.37%)to those of ZYMV-WS.In addition,the phylogenetic analysis of ZYMV revealed that all the isolates were clustered into two major branches,and its population structure assessment demonstrated a marked genetic differentiation between A4 and B.Furthermore,we also discovered the sites of specific amino-acid alteration,and their possible association with functional changes.These results will help to better understand the genetic diversity and phylogenetic process of ZYMV,and lay a theoretical foundation for the effective prevention and control of ZYMV infection in the near future.

关 键 词：小西葫芦黄花叶病毒 系统发育 多样性分析 选择压 

分 类 号：S432.41[农业科学—植物病理学]