青藤碱增加配对免疫球蛋白受体B表达抑制巨噬细胞经典活化  

Sinomenine promotes paired immunoglobulin-like receptor B expression to restrain macrophage classic activation

作　　者：卫智权[1] 包传红[1] 陈仪新 阎莉[1] WEI Zhi-quan;BAO Chuan-hong;CHEN Yi-xin;YAN Li(Guangxi University Key Laboratory of Basic and Applied Research on Zhuang Medical Prescriptions,GuangxiTraditional Chinese Medicine University,Nanning 530001,China;Guangxi Key Laboratory of Efficacy Studyon Chinese Materia Medica,Guangxi Traditional Chinese Medicine University,Nanning 530200,China)

机构地区：[1]广西中医药大学,广西高校壮医方药基础与应用研究重点实验室,广西南宁530001 [2]广西中医药大学,广西中药药效研究重点实验室,广西南宁530200

出　　处：《药学学报》2021年第6期1644-1652,共9页Acta Pharmaceutica Sinica

基　　金：国家自然科学基金:资助项目(81460765);广西高校壮医方药基础与应用研究重点实验室开放课题[桂教科研(2014)6号zyfy201502]。

摘　　要：本研究以体外实验探讨青藤碱通过上调配对免疫球蛋白样受体B(paired immunoglobulin-like receptor B,PIR-B)的表达水平而抑制巨噬细胞的经典活化。以脂多糖与γ干扰素联合刺激建立巨噬细胞经典活化细胞模型。分别以实时荧光逆转录-聚合酶链反应与蛋白免疫印迹方法检测细胞PIR-B的基因与蛋白表达,以酶联免疫吸附测定法检测培养上清液肿瘤坏死因子α与白细胞介素8,流式细胞术检测M1巨噬细胞,激光扫描共聚焦显微镜观察PIR-B原位表达。结果显示,青藤碱显著增加PIR-B表达,明显减少M1巨噬细胞百分比,降低培养上清液肿瘤坏死因子α、白细胞介素8水平。以上结果提示,青藤碱可显著抑制巨噬细胞经典活化,其机制可能与其增加巨噬细胞的PIR-B表达有关,此药理作用有助于解释青藤碱治疗类风湿性关节炎的药效机制。In this study,in vitro experiments were conducted to investigate that sinomenine inhibits the macrophage classic activation by up-regulating the expression of paired immunoglobulin-like receptor B(PIR-B).A macrophage model with classic activation was established by lipopolysaccharide and interferon-gamma co-stimulation.Real-time fluorescence reverse transcription-polymerase chain reaction was executed for evaluating the PIR-B gene expression,and Western blot for PIR-B protein expression,in macrophages,respectively.The tumor necrosis factorαand interleukin 8 in cell culture supernatant were measured by enzyme-linked immunosorbent assay.The flow cytometry was utilized to detect M1 macrophages.The PIR-B expression in situ was observed by laser scanning confocal microscope.The results showed that sinomenine significantly increased the expression of PIR-B,markedly reduced the percentage of M1 macrophages,and decreased the levels of tumor necrosis factorαand interleukin 8 in the culture supernatant.The above results indicated that sinomenine can significantly inhibit the macrophage classic activation,and its mechanism may be related to the increase of PIR-B expression in macrophages.This pharmacological effect helps explain the pharmacodynamic mechanism of sinomenine in treating rheumatoid arthritis.

关 键 词：青藤碱 类风湿性关节炎 配对免疫球蛋白受体B 巨噬细胞经典活化 M1巨噬细胞 

分 类 号：R285[医药卫生—中药学]