椎间盘组织冷冻切片制作技术的应用  

Application of Frozen Section Technology of Intervertebral Disc Tissue

作　　者：吴拮 王栋 李静 赵成相 WU Jie;WANG Dong;LI Jing;ZHAO Cheng-xiang(Institute of Orthopedics,Xijing Hospital,Air Force Military Medical University,Xi'an 710032,Shaanxi,China)

机构地区：[1]空军军医大学西京医院骨科研究所,陕西西安710032

出　　处：《医学信息》2021年第14期172-174,共3页Journal of Medical Information

摘　　要：目的探讨椎间盘组织冷冻切片在椎间盘退变相关研究中的优势。方法选取12周龄大鼠尾椎作为椎间盘样本来源,通过48 h的4%多聚甲醛固定和4周10%EDTA脱钙液脱钙处理后,行蔗糖梯度脱水以及OCT包埋切片。对切片进行组织学染色和免疫荧光染色。结果HE染色和番红O染色切片显示,椎间盘结构完整,整个髓核组织清淅可见,纤维环的结构相对完整,软骨终板的软骨样结构和椎体的骨性结构区分明显;HE染色和番红O染色切片显示,椎间盘结构完整,整个髓核组织清淅可见,纤维环的结构相对完整,软骨终板的软骨样结构和椎体的骨性结构区分明显。结论不论是常见的化学染色还是免疫荧光染色,冷冻切片均显示出对椎间盘组织较好的染色效果,但冷冻切片在椎间盘退变的基础研究中具有显著的优势。Objective To explore the advantages of frozen section of intervertebral disc tissue in the research of intervertebral disc degeneration.Methods The caudal vertebrae of 12-week-old rats were selected as the source of intervertebral disc samples.After 48 h of 4%paraformaldehyde fixation and 4 weeks of 10%EDTA decalcification treatment,sucrose gradient dehydration and OCT embedded sections were performed.Histological staining and immunofluorescence staining were performed on the sections.Results HE staining and safranin O staining showed that the structure of the intervertebral disc was complete,the whole nucleus pulposus tissue was clear,the structure of the annulus fibrosus was relatively complete,and the cartilage like structure of the cartilage endplate and the bone structure of the vertebral body were obviously distinguished;He staining and safranin O staining showed that the structure of intervertebral disc was complete,the whole nucleus pulposus tissue was clear,the structure of annulus fibrosus was relatively complete,and the cartilage like structure of cartilage endplate and bone structure of vertebral body were obviously distinguished.Conclusion No matter common chemical staining or immunofluorescence staining,frozen sections show good staining effect on intervertebral disc tissue,but frozen sections have significant advantages in basic research of intervertebral disc degeneration.

关 键 词：椎间盘组织 冷冻切片 染色 

分 类 号：Q2[生物学—细胞生物学] R681.53[医药卫生—骨科学]