植物丝氨酸羧肽酶样酰基转移酶的结构、功能和应用  

Structure, function and application of serine carboxypeptidase-like acyltransferases from plants

作　　者：王宇 杨燕[1] 刘忞之 王伟[1] Yu Wang;Yan Yang;Minzhi Liu;Wei Wang(State Key Laboratory of Bioactive and Function of Natural Medicines&Key Laboratory of Biosynthesis of Natural Products of National Health Commission of the People’s Republic of China,Institute of Materia Medica,Chinese Academy of Medical Science and Peking Union Medical College,Beijing 100050,China)

机构地区：[1]中国医学科学院北京协和医学院药物研究所,天然药物活性物质与功能国家重点实验实验室/国家卫生健康委员会天然药物生物合成重点实验室,北京100050

出　　处：《生物工程学报》2021年第6期1887-1899,共13页Chinese Journal of Biotechnology

基　　金：中国医学科学院医学与健康科技创新工程项目(No.2016-I2M-3-012);国家自然科学基金(Nos.81903487,82073757)资助;北京协和医学院合成生物学学科建设专项(No.201920100801)资助。

摘　　要：植物丝氨酸羧肽酶样酰基转移酶与丝氨酸羧肽酶相比具有相似的结构特点和很高的同源性,能够催化酰基葡萄糖酯的酰基转移反应,参与植物次生代谢产物的酰基化修饰,丰富天然产物结构多样性,改善化合物水溶性、稳定性等理化性质。本文重点介绍植物来源丝氨酸羧肽酶样酰基转移酶家族的结构特点、催化机制、功能鉴定及其生物催化应用等方面的研究进展,为促进此类酰基转移酶基因的功能表征和利用合成生物技术合成活性次生代谢产物提供参考。Plant serine carboxypeptidase-like acyltransferases(SCPL-AT) have similar structural characteristics and high homology compared to the serine carboxypeptidase. They can transfer the acyl from acyl glucose esters to many natural products, participate in the acylation modification of plant secondary metabolites, enrich the structural diversity of natural products, and improve the physicochemical properties such as water solubility and stability of compounds. This review summarizes the structural characteristics, catalytic mechanism, functional characterization, and biocatalytic applications of SCPL-AT from plants. This will help to promote the functional characterization of these acyltransferase genes and the biosynthesis of useful plant secondary metabolites by synthetic biotechnology.

关 键 词：丝氨酸羧肽酶样酰基转移酶 酰基化反应 双功能酰基和糖基供体 生物催化 

分 类 号：Q946.5[生物学—植物学]