Effects of inorganic carbon concentration and pH on carbonic anhydrase activity of gametophytes of Saccharina japonica  

作　　者：Yan-Hui Bi Cui-Ling Liang Jia-Li Li Hao Yin Ruo-Tong Tian Zhi-Gang Zhou 

机构地区：[1]Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources(Shanghai Ocean University),Ministry of Education,Shanghai,201306,China [2]International Research Center for Marine Biosciences at Shanghai Ocean University,Ministry of Science and Technology,Shanghai,201306,China [3]National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai,201306,China

出　　处：《Aquaculture and Fisheries》2021年第1期51-55,共5页渔业学报（英文）

基　　金：This research was supported by the National Key R&D Program of China(Grant No.2018YFD0901500);the National Natural Science Foundation of China(Grant No.41376136);the Double First-Class Discipline of Fisheries Science of China.

摘　　要：Carbonic anhydrase(CA)was considered to be an important component of carbon concentrating mechanism(CCM)of algae.It was an inducible enzyme.Environmental factors,especially dissolved inorganic carbon and pH,were known to affect CA activity.Effects of inorganic carbon(CO_(2)and HCO_(3)^(-))and pH on CA activity of gametophytes of Saccharina japonica were evaluated in this study.Under high-CO_(2)condition(3%CO_(2)),the activity of external CA(C_(Aext))was significantly decreased(P<0.05)from 33.92 REA/g FW to 27.69 REA/g FW.In contrast,the internal CA(CA_(int))and total CA activities were elevated significantly(p<0.01)from 36.83 REA/g FW to 48.80 REA/g FW,and from 70.75 REA/g FW to 76.49 REA/g FW,respectively.Addition of an appropriate concentration of HCO_(3)^(-)to the medium,CA_(int)and total CA activities were promoted significantly,although the activity of C_(Aext)was inhibited significantly(P<0.05).In higher HCO_(3)^(-)concentration(up to 1680 mg/L),the activity of CA_(int)was promoted to 60.81REA/g FW,which was twice that of the control.Higher activities of CA_(int)and total CA were induced at low pH.At pH 6.0,the highest activities of CA_(int)(110.85 REA/g FW)and total CA(128.17 REA/g FW)were induced.However,at pH 9.0,the activities of CA_(int)and total CA were reduced to the lowest of 23.31REA/g FW and 42.19 REA/g FW,respectively.This is the first report about the successful detection of C_(Aext)activity of gametophytes of S.japonica.The results would provide data for the analysis of Ci acquisition and transport mechanism in S.japonica gametophytes.

关 键 词：Saccharina japonica GAMETOPHYTES Carbonic anhydrase Inorganic carbon PH 

分 类 号：S917.4[农业科学—水产科学]