IFN-a production by human mononuclear cells infected with varicella-zoster virus through TLR9-dependent and-independent pathways  

作　　者：Hong-Ren Yu Hsin-Chun Huang Ho-Chang Kuo Jiunn-Ming Sheen Chia-Yo Ou Te-Yao Hsu Kuender D Yang 

机构地区：[1]Department of Pediatrics,Chang Gung Memorial Hospital-Kaohsiung Medical Center,Graduate Institute of Clinical Medical Science,Chang Gung University College of Medicine,Taiwan [2]Department of Obstetrics,Chang Gung Memorial Hospital-Kaohsiung Medical Center,Graduate Institute of Clinical Medical Science,Chang Gung University College of Medicine,Taiwan

出　　处：《Cellular & Molecular Immunology》2011年第2期181-188,共8页中国免疫学杂志（英文版）

基　　金：grants NSC 94-2314-B-182A-101(HR Yu);NSC 98-2314-B-182A-004-MY3(H R Yu)from the National Science Council,Taiwan.

摘　　要：Understanding the defense mechanisms of the host of an organism is important for infection control.In previous studies,we demonstrated that interferon-a(IFN-a),but not IL-12,was produced by human peripheral blood mononuclear cells infected with varicella-zoster virus(VZV).Here,we investigated what kind of cell(s)and which signal molecule(s)are involved in IFN-a production.Using cell isolation and ELISA,we found that plasmacytoid dendritic cells(pDCs)were responsible for IFN-a production during VZV infection.We also found that Toll-like receptor 9(TLR9)was involved in VZV-induced IFN-a production because inhibitory CpG oligodeoxynucleotide inhibited IFN-a production.UV-inactivated VZV-induced IFN-a production was lower than that of active VZV,indicating another TLR9-independent pathway.Further studies demonstrated that double-stranded RNA-dependent protein kinase,but not DNA-dependent protein kinase was involved in VZV-induced IFN-a production.Together,these results suggest that pDCs play an important role in IFN-a production during VZV infection through TLR9-dependent and-independent pathways.

关 键 词：IFN-A mononuclear cells plasmacytoid dendritic cell TLR9 varicella-zoster virus 

分 类 号：F42[经济管理—产业经济]