太湖土著田螺消化道中溶藻菌XMC溶藻进程与叶绿素a降解动力学研究  

Study on algae-lysing process and chlorophyll-a degradation kinetics of algicidal bacteria XMC in the digestive tract of indigenous field snails of Taihu Lake

作　　者：许明宸 张文艺 毛林强 XU Mingchen;ZHANG Wenyi;MAO Linqiang(Changzhou University,School of Environmental&Safety Engineering,Changzhou,213164,China)

机构地区：[1]常州大学环境与安全工程学院,常州213164

出　　处：《环境化学》2021年第6期1855-1861,共7页Environmental Chemistry

基　　金：水体污染控制与治理科技重大专项(2017ZX07202-004)资助.

摘　　要：在蓝藻爆发区域的太湖土著田螺消化道内,筛选出1株高效溶藻菌,命名为XMC.以铜绿微囊藻为受试对象,以叶绿素a含量检验溶藻菌XMC溶藻效果,考察了菌株溶藻能力、溶藻过程及其溶藻产物等.实验结果表明,溶藻菌XMC具有较强的溶藻能力,其生长曲线呈“S”型增长,符合Logistic动力学模型.菌藻共生环境中,藻液叶绿素a含量与溶藻时间两者之间符合一级动力学模型[Chla]=4831.82071×e^(−0.0241t),该模型可用于预测铜绿微囊藻溶藻过程的降解效果;溶藻菌XMC的溶藻进程以间接溶藻为主,通过分泌耐高温的非蛋白类溶藻物质来裂解藻细胞,菌液处理完的铜绿微囊藻液中,产物主要为藻细胞破裂分解出的芳环结构的氨基酸、酰胺类等物质.A high-efficiency algicidal bacteria named XMC was selected from the native snails of Taihu Lake,which lived in the area of cyanobacteria outbreak for a long time.Microcystis aeruginosa was used as the test object,and the chlorophyll-a content was used to test the algae-lysing effect of the algicidal bacteria XMC,and the algae dissolving ability,process and products of the bacteria were investigated.The experimental results showed that the algicidal bacteria XMC had strong algicidal ability,and the growth curve of algicidal bacteria XMC showed an“S”shape which accords with the Logistic dynamic model.The relationship between chlorophyll-a content and algae lysis time in the symbiotic environment of bacteria and algae conformed to the first-order kinetic model([Chla]=4831.82071×e^(−0.0241t)),which could be used to predict the degradation effect of Microcystis aeruginosa.The algicidal bacteria XMC was mainly an indirect algae lysate,which could lyse algal cells by secreting heat-resistant nonprotein lysates.In the treated Microcystis aeruginosa,the products were mainly amino acids and amides with aromatic ring structure broken down by algal cells.

关 键 词：溶藻菌 铜绿微囊藻 动力学模型 叶绿素A 

分 类 号：X52[环境科学与工程—环境工程] X172