两种水生昆虫头宽与基于图像分析的大小参数关系性分析  

Correlations Between Size Parameters Obtained Based on Image Analysis and Head Width of Two Species of Aquatic Insects

作　　者：李先福 陆永林 石欣 敖偲成 谭路[1] 叶麟[1] 蔡庆华[1,5] LI Xian-fu;LU Yong-lin;SHI Xin;AO Si-cheng;TAN Lu;YE Lin;CAI Qing-hua(State Key Laboratory of Freshwater Ecology and Biotechnology,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,China;Institute of Eastern-Himalaya Biodiversity Research,Dali University,Dali 671003,China;Collaborative Innovation Center for Biodiversity and Conservation in the Three Parallel Rivers Region of China,Dali University,Dali 671003,China;The Key Laboratory of Yunnan Education Department on Er'hai Catchment Conservation and Sustainable Development,Dali 671003,China;University of Chinese Academy of Sciences,Beijing 100049,China)

机构地区：[1]中国科学院水生生物研究所淡水生态与生物技术国家重点实验室,湖北武汉430072 [2]大理大学东喜玛拉雅研究院,云南大理671003 [3]中国三江并流区域生物多样性协同创新中心,云南大理671003 [4]云南省高校洱海流域保护与可持续发展研究重点实验室,云南大理671003 [5]中国科学院大学,北京100049

出　　处：《长江流域资源与环境》2021年第6期1466-1471,共6页Resources and Environment in the Yangtze Basin

基　　金：国家重点研发计划项目(2017YFC0506406);中国科学院战略先导A类项目(XDA23080101);国家自然科学基金项目(31960255)。

摘　　要：对于水生昆虫及其它生物而言,大小是一个重要的功能性状,但是头宽的测量特别耗时和受人为因素影响。随着计算机技术的发展,基于图像分析技术(一种快速测量大小的策略)可获得传统方式难以获得的大小参数,明确这些大小参数与头宽的关系,对推动这些参数的应用具有重要意义。选择两种个体大小差异较大的蜉蝣(梧州蜉和光滑细蜉)作为研究对象,对其头宽与基于数字图像获得的面积,周长,最小矩形的长和宽,最适合椭圆的长轴和短轴和粒径等个体大小参数的相关性进行研究。数字图像采用改进后的描述仪扫描获取;图像分析基于ImageJ软件完成;基于二元直线回归和多元直线回归分析各参数之间的相关性。各种层次的回归分析结果均表明:基于图像分析获得的两种蜉蝣所有个体大小参数均与头宽大小呈极其显著的正相关性,其中面积的相关性最高。并且面积与其它大小参数相关性也极其显著,对个体大小具有较好的代表性。研究结果为推动数字图像分析技术应用在水生昆虫及其它生物的生物学和生态学研究中提供了技术支持和理论依据。Head width is an important individual size parameter of insects, but the measurement of head width has some limitations, such as effects of human error, high time cost, and the use of a single parameter.With the development of computer technology, some size parameters of insects, including aquatic insects, which were previously difficult to ascertain, can now be obtained through image-based analysis.To promote the application of these size parameters in studies on phenology and age structure of insects, especially aquatic insects, it is important to clarify the correlations between size parameters and head width.Two species of aquatic insects, Ephemera wuchowensis Hsu, 1937 and Caenis lubrica Tong and Dudgeon, 2002,were selected for this study.These species have large differences in individual size throughout the larval period, and were selected to study the correlations between head width and individual size parameters obtained based on digital images, including area, perimeter, width and height of bounding rectangle, major and minor of fit ellipse, and Feret’s diameter.Digital images of the insects were obtained by scanning using a modified scanner, and the image analyses were carried out using ImageJ software.Correlation analysis of parameters based on binary linear regression and multivariate linear regression.The results show that all the individual size parameters of the two species of aquatic insects obtained based on ImageJ analysis had very high and significantly positive correlations with head width, and the correlation between area and head width was the most prominent.And the correlation between area and other size parameters is also very significant, which is a good representation of individual size.The results provide technical support and a theoretical basis for promoting the application of digital image analysis technology in biological and ecological studies on aquatic insects and other insects.

关 键 词：昆虫 体型大小 面积 图像分析 大小测量 IMAGEJ 

分 类 号：Q958.8[生物学—动物学]