RhizoVision Crown:An Integrated Hardware and Software Platform for Root Crown Phenotyping  

作　　者：Anand Seethepalli Haichao Guo Xiuwei Liu Marcus Griffiths Hussien Almtarfi Zenglu Li Shuyu Liu Alina Zare Felix B.Fritschi Elison B.Blancaflor Xue-Feng Ma Larry M.York 

机构地区：[1]Noble Research Institute,LLC,2510 Sam Noble Parkway,Ardmore,OK 73401,USA [2]Division of Plant Sciences,University of Missouri,Columbia,MO 65201,USA [3]Crop and Soil Sciences,University of Georgia,Athens,GA 30602,USA [4]Texas A&M AgriLife Research,Texas A&M University System,Amarillo,TX 79106,USA [5]Department of Electrical and Computer Engineering,University of Florida,Gainesville,FL 32601,USA

出　　处：《Plant Phenomics》2020年第1期25-39,共15页植物表型组学（英文）

基　　金：The work was funded by the Noble Research Institute,LLC;the USDA NIFA EAGER program(2017-67007-26953);the Department of Energy ARPA-E ROOTS program(DE-AR0000822);the United Soybean Board(1420-532-5613).

摘　　要：Root crown phenotyping measures the top portion of crop root systems and can be used for marker-assisted breeding,genetic mapping,and understanding how roots influence soil resource acquisition.Several imaging protocols and image analysis programs exist,but they are not optimized for high-throughput,repeatable,and robust root crown phenotyping.The RhizoVision Crown platform integrates an imaging unit,image capture software,and image analysis software that are optimized for reliable extraction of measurements from large numbers of root crowns.The hardware platform utilizes a backlight and a monochrome machine vision camera to capture root crown silhouettes.The RhizoVision Imager and RhizoVision Analyzer are free,open-source software that streamline image capture and image analysis with intuitive graphical user interfaces.The RhizoVision Analyzer was physically validated using copper wire,and features were extensively validated using 10,464 groundtruth simulated images of dicot and monocot root systems.This platform was then used to phenotype soybean and wheat root crowns.A total of 2,799 soybean(Glycine max)root crowns of 187 lines and 1,753 wheat(Triticum aestivum)root crowns of 186 lines were phenotyped.Principal component analysis indicated similar correlations among features in both species.The maximum heritability was 0.74 in soybean and 0.22 in wheat,indicating that differences in species and populations need to be considered.The integrated RhizoVision Crown platform facilitates high-throughput phenotyping of crop root crowns and sets a standard by which open plant phenotyping platforms can be benchmarked.

关 键 词：CROWN utilize intuitive 

分 类 号：S126[农业科学—农业基础科学]