杨树响应细菌性溃疡病菌侵染的miRNA靶基因预测及分析  

Prediction and Analysis of miRNA Targets in Poplar in Response to the Infection of Lonsdalea quercina subsp. Populi

作　　者：徐千惠 常福利 霍晓薇 周科 王延伟 Xu Qianhui;Chang Fuli;Huo Xiaowei;Zhou Ke;Wang Yanwe(Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plant of Ministry of Education,National Engineering Laboratory for Tree Breeding,Beijing Advanced Innovation Center for Tree Breeding by Molecular Design,Beijing Forestry University,Beijing,100083;Jiaozuo Administration,Taihang Mountains National Nature Reserve,Jiaozuo,454000)

机构地区：[1]北京林业大学林木分子设计育种高精尖创新中心林木育种国家工程实验室林木花卉遗传育种教育部重点实验室,北京100083 [2]焦作管理局河南太行山国家级自然保护区,焦作454000

出　　处：《分子植物育种》2021年第12期3959-3972,共14页Molecular Plant Breeding

基　　金：国家自然科学基金(31670671,31470668)资助。

摘　　要：欧美杨细菌性溃疡病是由Lonsdalea quercina subsp.Populi引起的枝干病害,给杨树的生长和生存带来极大危害。miRNA是植物响应逆境胁迫的重要调控分子,为鉴定杨树中响应欧美杨细菌性溃疡病菌侵染的miRNA靶基因,以期为林木抗病分子育种提供新的候选基因,本研究以miRBase数据库中杨树全部miRNA成熟序列为探针,对‘中林46’杨接种细菌性溃疡病后的差异表达基因进行了miRNA的靶基因预测。共筛选出杨树127个miRNA家族的276个miRNA对应的566个靶基因,涉及植物病原互作、植物激素信号传导和苯丙素生物合成等多个途径,表明miRNA参与了杨树对细菌性溃疡病菌侵染的响应。结合靶基因的GO分析、KEGG通路富集和基因功能注释,发现miR482、miR6459、miR7812、miR7835和miR396等通过靶定RPS2、NBS-LRR、AUX1、CCR、Ca2+跨膜运输蛋白编码基因,参与调控杨树对病菌侵染的响应。miR7835靶定的9个差异表达基因中有6个基因均编码苯丙素生物合成途径通路中的CCR酶,推测其可能影响杨树木质素合成,进而参与杨树对病菌侵染的防御反应。本研究发现的miRNA:mRNA调控基因对,丰富了林木抗病分子育种的基因资源,为林木抗病分子调控机制的研究提供了新的线索。Euramerican poplar bacterial canker disease caused by Lonsdalea quercina subsp.Populi,is one of the stem diseases and seriously harmful to the growth and survival of poplar.miRNAs play crucial roles in the regulation of the stress response of plants.To identify target genes of miRNAs responsive to the infection of this pathogen and provide candidate genes to the molecular breeding of forest disease resistance,the mature sequences of poplar miRNAs in miRBase database were used as probes to in silico predict the differentially expressed targets in poplar’Zhonglin 46’inoculated with bacterial canker by using psRNATarget in this investigation.A total of 566 target genes of 276 miRNAs belonging to 127 miRNA families were screened,and these targets were found to be involved in multiple pathways such as plant pathogen interaction,plant hormone signaling,and phenylpropanoid biosynthesis,suggesting that these miRNAs were involved in the regulation of the response to L.quercina infection.Combined with GO analysis,KEGG pathway enrichment and gene function annotation of target genes,it was showed that miRNAs including miR482,miR6459,miR7812 and miR7835 were predicted to target genes encoding RPS2,NBS-LRR,AUX1,CCR,and Ca2+transmembrane transport protein genes,which were involved in the resistance regulation of poplar to L.quercina infection.Six of the nine differentially expressed genes targeted by miR7835 encode the CCR enzyme in the phenylpropanol biosynthesis pathway,which might be involved in the defense response of poplars by affecting the synthesis of lignin.The miRNA:mRNA regulatory gene pairs found in this investigation enlarged the gene resources of molecular breeding of forest disease resistance,and provided new clues for the research of the molecular regulatory mechanism of disease resistance in trees.

关 键 词：杨树 MIRNA 靶基因 欧美杨细菌性溃疡病菌 

分 类 号：S763.7[农业科学—森林保护学]