利用QTL-seq挖掘与结球甘蓝叶柄长度相关的基因  

Using QTL-seq to Mine Genes Related to Petiole Length of Cabbage

作　　者：邰翔[1] 朱晓炜 陈锦秀[1] 顾大国 薄天岳[1] Tai Xiang;Zhu Xiaowei;Chen Jinxiu;Gu Daguo;Bo Tianyue(Horticultural Research Institute,Shanghai Academy of Agricultural Sciences,Shanghai,201106;Shanghai Xinghui Vegetables Company Limited,Shanghai,201419)

机构地区：[1]上海市农业科学院园艺研究所,上海201106 [2]上海星辉蔬菜有限公司,上海201419

出　　处：《分子植物育种》2021年第12期4063-4069,共7页Molecular Plant Breeding

基　　金：“十三五”国家科技重点研发计划项目(2016YFD0101702);上海市科技兴农攻关项目(沪农科攻字[2015]第6-1-7号);国家现代农业产业技术体系建设专项资金项目(CARS-25)共同资助。

摘　　要：结球甘蓝外叶叶柄长度决定其单株占地面积,挖掘与结球甘蓝外叶叶柄长度相关的基因对于提高结球甘蓝的定植密度和单位面积产量意义重大。本试验利用结球甘蓝长叶柄高代自交系‘294’(母本)、短叶柄高代自交系‘301’(父本)及其F2群体为材料,从F2中选出了极端性状植株(长叶柄,短叶柄各30株)构建了DNA混池,利用QTL-seq与SNP/InDel-index相结合的分析方法得到与甘蓝外叶叶柄长度相关的候选基因。本研究从甘蓝全基因组中挖掘出6个控制甘蓝叶柄长度的候选基因,这些基因分别富集在SKU5蛋白编码、XTH蛋白编码及植物细胞壁合成的3条通路上。该研究为结球甘蓝叶柄长度相关基因的克隆及其功能分析提供了参考依据。The length of the petiole determines the area of a single plant.It is great significance to mining genes related to the length of the petiole of cabbage.In this experiment,short-petiole of high-bred line’301’(male parent),long-petiole of high-bred line’294’(female parent)and its F2 were used as materials.Extreme trait materials were selected from F2(30 long-petiole and 30 short-petiole materials)were used to construct pools of DNA.The candidate genes related to the petiole length of cabbage were mining using combination of QTL-seq and SNP/InDel-index.In this study,six candidate genes controlling the petiole length were identified from genome of cabbage.Seven candidate genes were enriched in the three pathways of SKU5 protein coding,XTH protein coding and plant cell wall synthesis.This study provides a reference for cloning and functional analysis of genes related to petiole length in cabbage.

关 键 词：结球甘蓝 叶柄长度 QTL-seq 

分 类 号：S635.1[农业科学—蔬菜学]