基于SRAP标记分析的雄性榧树及榧树物种的遗传多样性  

Genetic Diversity of the Male and Species in Torreya grandis Based on SRAP Analysis

作　　者：刘浩凯 张辉[1,3] 陈文充 喻卫武 戴文圣[1] 曾燕如[1] Liu Haokai;Zhang Hui;Chen Wenchong;Yu Weiwu Dai;Wensheng;Zeng Yanru(The State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou,311300;Jingning Bureau of Natural Resources and Planning,Jingning She Autonomous County,Jingning,323500;Jingning Ecological and Forestry Development Center,Jingning She Autonomous County,Jingning,323500)

机构地区：[1]浙江农林大学省部共建亚热带森林培育国家重点实验室,杭州311300 [2]浙江省景宁畲族自治县自然资源和规划局,景宁323500 [3]浙江省景宁畲族自治县生态林业发展中心,景宁323500

出　　处：《分子植物育种》2021年第12期4153-4158,共6页Molecular Plant Breeding

基　　金：浙江农林大学省部共建亚热带森林培育国家重点实验室项目(ZY20180208);浙江省科技厅重点研发项目(2018C02004)共同资助。

摘　　要：雌雄异株天然杂交的榧树(Torreya grandis Fort. ex Lindl)存在着丰富的自然变异,具有巨大的选育潜力,自然界也不乏与榧树优良自然变异类型经无性繁殖培育而成的栽培类型香榧(T. grandis‘Merrilli’)相媲美的优良种质。但由于历史的原因及香榧产业的发展,榧树的遗传多样性受到了一定的影响。本研究在已有雌性榧树研究的基础上,采用SRAP标记对雌性榧树相同采样地的雄性榧树居群进行分析,并整合雌雄榧树居群的标记分析结果,研究了榧树物种水平的遗传多样性。结果表明,雄性榧树居群平均PPL为63.67%,等位基因数(Na)为1.636 7,有效等位基因数(Ne)为1.381 3,Nei's基因多样性(H)为0.221 8,Shannon信息指数(I)为0.331 1,说明雄性榧树存在较为丰富的遗传多样性,且变异(81.48%)主要存在于居群内个体间;与雌性榧树遗传参数相比,雄性榧树的相关遗传参数均不如雌株;就物种水平而言,榧树丰富的遗传多样性来源于雄、雌榧树共同的作用。基于研究结果提出要注重保护榧树尤其是雄性榧树的遗传多样性,同时建议基于个体的优良表现开展优株选择。Torreya grandis Fo rt.ex Li ndl,a dioecious species,has a multitude of natural variation and great potential for selection and breeding.Naturally there exist some superior individuals which could be comparable to T.grandis’Merrillii’,an excellent cultivar vegetatively propagated from a superior natural variant from T.grandis.Development of T.grandis’Merrillii’industry,in addition to some historical reasons,has some effects on genetic diversity of the species.This study analyzes SRAP marker-based genetic diversity of male populations from the same sampling sites as the female populations and genetic diversity at the species level in T.grandis with the maker data from both female and male populations.The result showed that male T.grandis populations had an abundant genetic diversity with a percentage of polymorphic loci(PPL)of 63.67%,a number of alleles(Na)of1.6367,an effective number of alleles(Ne)of 1.3813,a Nei’s gene diversity(H)of 0.2218,and a Shannon Information index(I)of 0.3311 and 81.48%of genetic variation existed among individuals within a population.Male T.grandis populations were not better than female populations in terms of genetic parameters.Both the male and female acted on the rich genetic diversity of T.grandis at the species level.Based on the results of this study,it is suggested that importance be attached to the conservation of genetic diversity in T.grandis,especially that of the male population,and selection of superior individuals could be conducted based on their good performance.

关 键 词：榧树(Torreya grandis) SRAP 遗传多样性 

分 类 号：S791.53[农业科学—林木遗传育种]