重组禽流感病毒H5亚型三价灭活疫苗对商品蛋鸡免疫程序优化  

Optimization of Immune Procedure for Commercial Layers with Trivalent Inactivated Vaccine of H5 Subtype of Recombinant Avian Influenza Virus

作　　者：王申锋[1] 钱明珠[1] 谢军强 王德刚 朱红标 胡梅[1] 化军[1] WANG Shen-feng;QIAN Ming-zhu;XIE Jun-qiang;WANG De-gang;ZHU Hong-biao;HU Mei;HUA Jun(College of Animal Husbandry Engineering,Henan Agricultural Vocational College,Zhengzhou 451450,China;Social Affairs Bureau of Zhengzhou Airport Economic Comprehensive Experimental Zone,Zhengzhou 450019,China;Animal Husbandry and Veterinary Station of Tangyin County,Tangyin 456150,China;Henan Jianheng Animal Pharmaceutical Limited Company,Zhengzhou 451476,China)

机构地区：[1]河南农业职业学院牧业工程学院,河南郑州451450 [2]郑州航空港经济综合试验区社会事业局,河南郑州450019 [3]汤阴县畜牧兽医总站,河南汤阴456150 [4]河南健恒动物药业有限公司,河南郑州451476

出　　处：《黑龙江农业科学》2021年第7期48-51,共4页Heilongjiang Agricultural Sciences

基　　金：河南农业职业学院科研创新团队项目(HNACKT-2020-3)。

摘　　要：为优化商品蛋鸡场禽流感的免疫程序,掌握商品蛋鸡场H5亚型禽流感母源抗体的消长规律和不同首免日龄免疫禽流感病毒H5亚型三价灭活疫苗(Re-6株+Re-7株+Re-8株)疫苗的抗体效价消长情况,本试验将商品蛋鸡分成5组,1~4组为试验组,分别于7、10、14和21日龄免疫禽流感病毒H5亚型三价灭活疫苗,第5组为空白对照组,于14日龄肌注生理盐水,然后进行禽流感抗体检测。结果表明:蛋鸡2日龄时母源抗体水平最高,平均效价都在6 log2以上,且群体抗体合格率都在99%以上。随后,逐渐下降,在10日龄时处在6 log2附近,这表明鸡群面临禽流感病毒感染风险较大;首免日龄在7日龄、10日龄时,接种疫苗到鸡只产生具有保护作用的抗体大概在14~17 d,此间母源抗体水平也较低,疫苗刺激机体产生的抗体不足以保护鸡只。如果首免日龄在14日龄以后则更不利于鸡群禽流感的防控。建议该蛋鸡场雏鸡H5亚型禽流感疫苗首免时间在7日龄最佳。In order to optimize the immunization procedure of avian influenza in commercial layer farms,master the growth and decline rule of maternal antibody against H5 subtype avian influenza in commercial layer farms and the antibody titer of trivalent inactivated vaccine(Re-6 strain+Re-7 strain+Re-8 strain)against H5 subtype avian influenza virus at different first immunization days.The commercial layers were divided into 5 groups in this experiment.Groups 1-4 were immunized with trivalent inactivated vaccine of H5 subtype of avian influenza virus at 7,10,14 and 21 days of age,respectively.Group 5 was the blank control group,and the normal saline was injected intramuscularly at 14 days of age,and then avian influenza antibody was detected.The results showed that the maternal antibody level of laying hens was the highest at the age of 2 days,the average titer was more than 6 log2,and the qualified rate of group antibody was more than 99%.Then,it decreased gradually,and it was near 6 log2 at the age of 10 days,which indicated that the chickens were at high risk of avian influenza virus infection;when the first immunization age was 7 days and 10 days,the protective antibody produced by the vaccinated chickens was about 14-17 d,and the maternal antibody level was also low.The antibody produced by the vaccinated chickens was not enough to protect chickens.If the first immunization age is after 14 days,it will be more unfavorable for the prevention and control of avian influenza in chickens.We suggested that the first immunization time of H5 subtype avian influenza vaccine should be 7 days old.

关 键 词：禽流感H5亚型三价灭活疫苗 商品蛋鸡 抗体水平 免疫程序 

分 类 号：S85[农业科学—兽医学]