基于地标点法的绥芬河绒螯蟹种群鉴定  

Landmark-Based Morphometric Identification of Mitten Crab Eriocheir from Suifen River

作　　者：姜晓东[1,2,3] 成永旭 杨雨虹[3] 吴旭干[1,2,4] JIANG Xiaodong;CHENG Yongxu;YANG Yuhong;WU Xugan(Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of Agriculture and Rural Affairs, Shanghai Ocean University, Shanghai 201306, China;Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding, Shanghai Ocean University, Shanghai 201306, China;College of Animal Science and Technology, Northeast Agricultural University, Harbin 150036, China;National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai 201306, China)

机构地区：[1]上海海洋大学,农业农村部淡水种质资源重点实验室,上海201306 [2]上海海洋大学,水产动物遗传育种上海市协同创新中心,上海201306 [3]东北农业大学动物科学技术学院,黑龙江哈尔滨150036 [4]上海海洋大学,水产科学国家级实验教学示范中心,上海201306

出　　处：《水产科学》2021年第4期501-507,共7页Fisheries Science

基　　金：科技部蓝色粮仓项目(2018YFD0900103,2018YFD0900603);财政部和农业农村部现代农业产业技术体系专项(CARS-48);上海市高水平地方高校建设科研项目(A1-2801-18-1003).

摘　　要：为探究绥芬河流域野生绒螯蟹的种群现状,2017年9—11月和2018年10月在黑龙江省牡丹江市、江苏省镇江市、广西北海市和日本兵库县的天然水域中各采集野生雌、雄绒螯蟹样本各15只,运用基于地标点法的几何形态测量学方法比较绥芬河野生绒螯蟹与以长江水系为代表的中华绒螯蟹,以及与以日本本土和我国合浦水域为代表的日本绒螯蟹的形态特征差异。研究结果表明:(1)主成分散点图显示,长江与绥芬河群体部分重叠并形成较为集中的一块区域;日本与合浦群体形成另外一块较为集中的区域;(2)网格变形图显示,各群体头胸甲的形态差异主要为额刺和侧刺长度;(3)逐步判别分析中绥芬河和长江群体仅个别样本相互误判,判别准确率为87%~93%,而合浦和日本水域野生绒螯蟹的判别准确率均为100%;(4)聚类分析先将长江和绥芬河群体聚为一支,然后将合浦和日本本土群体聚为另外一支。综上,以头胸甲为研究对象的地标点几何形态测量法将绥芬河流域野生绒螯蟹归类为中华绒螯蟹,但仍需要结合分子生物学技术加以辅助证实。This study was conducted to compare the morphological characters of wild mitten crab Eriocheir collected from natural waters in Mudanjiang City,Heilongjiang Province,Zhenjiang City,Jiangsu Province,Beihai City,Guangxi Province,and Hyogo Prefecture in Japan,15 male and 15 female samples at each sampling site,including Suifen River(SF)population from September to November 2017 and October 2018 with the wild Chinese mitten crab Eriocheir sinensis in Changjiang River(YZ)as well as the wild Japanese mitten crab E.japonicus in the Hepu Basin(HP)and the Hyogo of Japan(JP)in order to explore the current status of the wild mitten crab Eriocheir population in the Suifen River Basin.(1)The principal component analysis showed that SF and YZ individuals were slightly overlapped and divided into an independent group by the first two principal components,and that HP and JP individuals were divided into another independent group.(2)The thin-plate splines showed that morphological differences between populations mainly focused on frontal and lateral spines.(3)The stepwise discriminant analysis revealed that discriminative accuracies of SF and YZ individuals were 87%—93%and that the discriminative accuracy of HP and JP individuals was both 100%.(4)In the cluster analysis,SF and YZ individuals were clustered firstly,whereas HP and JP individuals formed another distinct cluster.In conclusion,the wild mitten crab in the Suifen River had closer morphological characters to the Chinese mitten crab based on landmark-based morphometrics analysis,but it still needs to be confirmed by molecular biological technique.

关 键 词：绥芬河流域 中华绒螯蟹 日本绒螯蟹 地标点法 种群鉴别 

分 类 号：S932.52[农业科学—渔业资源]