急性高温胁迫对大口黑鲈“优鲈3号”组织损伤及HSPs基因表达的影响  

Effect of Acute High Temperature Stress on Tissue Damage and HSPs Gene Expression of Largemouth Bass Micropterus salmoides“Youlu No.3”

作　　者：陆健 张佳佳 周国勤 王佩佩 庆辉 LU Jian;ZHANG Jiajia;ZHOU Guoqin;WANG Peipei;QING Hui(Institute of Fisheries Sciences of Nanjing City, Nanjing 210036, China)

机构地区：[1]南京市水产科学研究所,江苏南京210036

出　　处：《水产科学》2021年第4期508-515,共8页Fisheries Science

基　　金：江苏省海洋与渔业局2018年渔业科技类项目(Y2018-1).

摘　　要：将25℃(对照组)下暂养的体质量(9.87±1.20)g大口黑鲈“优鲈3号”幼鱼直接放入28、31、34、37℃水环境中,0、6、48 h时取肝脏和鳃组织,探究不同温度应激后对大口黑鲈“优鲈3号”幼鱼肝脏、鳃组织结构损伤及HSPs基因在这些组织中表达的影响。试验结果显示,28℃和31℃组肝脏和鳃组织均与对照组无差异,34℃肝脏和鳃组织出现轻微的细胞水肿,37℃则出现肝细胞空泡化、细胞间界限杂乱模糊、细胞核溶解,鳃小片末端出现膨大且弯曲,部分鳃小片因红细胞过多而涨破。在不同水温下,HSPs基因的表达量变化不同。6 h时,肝脏和鳃组织中HSP70和HSC70 mRNA表达量均随着胁迫温度的升高而升高,在37℃时表达量最高;48 h时,肝脏和鳃组织中HSP70和HSC70 mRNA表达量相较于6 h均出现不同程度的下降(除31℃组鳃组织HSP70);且随着胁迫温度的升高呈先升后降的趋势,37℃组的表达量最低。结果表明,高温胁迫会使大口黑鲈“优鲈3号”幼鱼产生一定应激损伤,因此在实际养殖生产中,应密切关注温度的变化,降低温度胁迫对鱼体免疫机能的影响。Juvenile largemouth bass Micropterus salmoides“Youlu No.3”with body weight of(9.87±1.2)g held at 25℃(control group)was directly exposed to the water environment at 28,31,34,and 37℃and sampled at 0 h,6 h,and 48 h to investigate the effects of temperature on structural damage of liver and gill tissues and expression of HSPs gene in liver and gill of largemouth bass“Youlu No.3”.The results showed that no differences in structure of the liver and gill tissues were observed in the 28℃and 31℃groups between the treated and control largemouth bass“Youlu No.3”.However,there was slight cell edema in liver and gill tissues at 34℃,and the liver cells showed increase in vesicles structure,the messy and blurred,and the nucleus dissolved boundaries between cells at 37℃.Meanwhile,the gill lamellae became overall edema and severely bending,some swollen gill lamellae due to too many red blood cells.The expression levels of HSPs gene were found to be varied with changes in different water temperatures.At 6 h,the mRNA expression levels of HSP70 and HSC70 in liver and gill tissues were increased with the increase in stress temperature,with the maximal expression levels of HSP70 and HSC70 at 37℃.At 48 h,the mRNA expression levels of HSP70 and HSC70 in liver and gill tissues were decreased to some extent compared to that at 6 h(except for HSP70 at 31℃gill tissue).The mRNA expression levels of HSP70 and HSC70 in liver and gill tissues showed a trend of first increasing and then decreasing,with the minimal level in the 37℃group.The findings indicated that high temperature stress led to some stress damage to largemouth bass“Youlu No.3”.In aquaculture practices,attention should be paid to changes in temperature to reduce the impact of temperature stress on the immune function of fish.

关 键 词：大口黑鲈“优鲈3号” 急性高温胁迫 组织损伤 HSP70 HSC70 

分 类 号：S965.211[农业科学—水产养殖]