红尾仙女虾养殖群体ISSR反应体系建立及遗传多样性分析  

Establishment of ISSR Reaction System and Analysis of Genetic Diversity in Cultured Populations of Fair Shrimp Branchinella thailandensis

作　　者：谭舜 杨逸文 蒋小美 姜波[1] 徐建荣[1] 韩晓磊[1] TAN Shun;YANG Yiwen;JIANG Xiaomei;JIANG Bo;XU Jianrong;HAN Xiaolei(Changshu Institute of Technology,Changshu 215500,China;Nanjing Xianxia Biotechnology Co.,Ltd.,Nanjing 210000,China)

机构地区：[1]常熟理工学院,江苏常熟215500 [2]南京仙夏生物科技有限公司,江苏南京210000

出　　处：《水产学杂志》2021年第3期22-27,共6页Chinese Journal of Fisheries

基　　金：苏州市科技计划项目农业应用基础研究(SNG201915).

摘　　要：针对24尾养殖红尾仙女虾Branchinella thailandensis样本的25μL PCR体系,进行了Mg2+、引物浓度、Taq DNA聚合酶3因素3水平L9(34)正交实验;退火温度范围设置为48~59℃(间隔1℃),优化了ISSR反应体系及筛选的6个多态性和重复性较好的引物,分析了红尾仙女虾养殖群体的遗传多样性。结果共扩增出37个位点,其中32个多态位点,多态位点比率86.49%,Nei's指数和Shannon's指数分别为0.2570和0.3962,表明ISSR分子标记技术能够稳定可靠地应用于红尾仙女虾遗传多样性分析研究;红尾仙女虾养殖群体具有较高的遗传多样性,人工养殖环境对其遗传多样性的影响较小。The optimized ISSR reaction system including the concentration of Taq enzyme,Mg2+,and primers were investigated in breeding fairy shrimp Branchinella thailandensis using 3 factors and 3 levels orthogonal design.The optimized ISSR reaction system and the 6 selected primers with good polymorphism and repeatability were used to analyze the genetic diversity of 24 samples from the breeding population of B.thailandensis.A total of 37 loci were amplified,including 32 polymorphic loci,with polymorphic locus ratio of 86.49%,Nei's index of 0.2570 and Shannon's index of 0.3962.The findings indicated that ISSR molecular marker technique was applied stably and reliably to analyze the genetic diversity of B.thailandensis and that the breeding population of B.thailandensis had high genetic diversity,which showed little influence by artificial breeding environment.

关 键 词：红尾仙女虾 ISSR 反应体系 遗传多样性 

分 类 号：Q347[生物学—遗传学] S917[农业科学—水产科学]