Structural study of the Cdc25 domain from Ral-specific guanine-nucleotide exchange factor RalGPS1a  

作　　者：Wei Peng Jiwei Xu Xiaotao Guan Yao Sun Xuejun C.Zhang Xuemei Li Zihe Rao 

机构地区：[1]National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Protein & Cell》2011年第4期308-319,共12页蛋白质与细胞（英文版）

基　　金：the State Key Development Program for Basic Research of the Ministry of Science and Technology of China(973 Project)(Grant Nos.2007CB914304,2011CB915501,and 2011CB910304);the National High Technology Research and Development Program of China(863 Project)(Grant No.2006AA02A322);National Key Technologies R&D Program(Grant No.2009ZX10603)of the Ministry of Health of China.

摘　　要：The guanine-nucleotide exchange factor(GEF)RalGPS1a activates small GTPase Ral proteins such as RalA and RalB by stimulating the exchange of Ral bound GDP to GTP,thus regulating various downstream cellular processes.RalGPS1a is composed of an Nterminal Cdc25-like catalytic domain,followed by a PXXP motif and a C-terminal pleckstrin homology(PH)domain.The Cdc25 domain of RalGPS1a,which shares about 30%sequence identity with other Cdc25-domain proteins,is thought to be directly engaged in binding and activating the substrate Ral protein.Here we report the crystal structure of the Cdc25 domain of RalGPS1a.The bowl shaped structure is homologous to the Cdc25 domains of SOS and RasGRF1.The most remarkable difference between these three Cdc25 domains lies in their active sites,referred to as the helical hairpin region.Consistent with previous enzymological studies,the helical hairpin of RalGPS1a adopts a conformation favorable for substrate binding.A modeled RalGPS1a-RalA complex structure reveals an extensive binding surface similar to that of the SOS-Ras complex.However,analysis of the electrostatic surface potential suggests an interaction mode between the RalGPS1a active site helical hairpin and the switch 1 region of substrate RalA distinct from that of the SOS-Ras complex.

关 键 词：RalGPS1a RALA cdc25 domain crystal structure 

分 类 号：O62[理学—有机化学]