Spliceosomal genes in the D.discoideum genome:a comparison with those in H.sapiens,D.melanogaster,A.thaliana and S.cerevisiae  

作　　者：Bing Yu Petra Fey Karen E.Kestin-Pilcher Alexei Fedorov Ashwin Prakash Rex L.Chisholm Jane Y.Wu 

机构地区：[1]Department of Molecular and Clinical Genetics,Royal Prince Alfred Hospital and Sydney Medical School(Central),the University of Sydney,NSW 2006,Australia [2]dictyBase,Center for Genetic Medicine,Northwestern University,Chicago,IL 60611,USA [3]Department of Neurology and Lurie Comprehensive Cancer Center,Center for Genetic Medicine,Northwestern University Feinberg Medical School,Chicago,IL 60611,USA [4]Department of Medicine and Program in Bioinformatics and Proteomics/Genomics,The University of Toledo,Toledo,OH 43614,USA

出　　处：《Protein & Cell》2011年第5期395-409,共15页蛋白质与细胞（英文版）

基　　金：supported by grants to J.Y.W from NIH(EY014576 and GM070967);A.F.from NSF Career award MCB-0643542 and to R.L.C.from NIH(GM64426 and HG02273).

摘　　要：Little is known about pre-mRNA splicing in Dictyostelium discoideum although its genome has been completely sequenced.Our analysis suggests that pre-mRNA splicing plays an important role in D.discoideum gene expression as two thirds of its genes contain at least one intron.Ongoing curation of the genome to date has revealed 40 genes in D.discoideum with clear evidence of alternative splicing,supporting the existence of alternative splicing in this unicellular organism.We identified 160 candidate U2-type spliceosomal proteins and related factors in D.discoideum based on 264 known human genes involved in splicing.Spliceosomal small ribonucleoproteins(snRNPs),PRP19 complex proteins and late-acting proteins are highly conserved in D.discoideum and throughout the metazoa.In non-snRNP and hnRNP families,D.discoideum orthologs are closer to those in A.thaliana,D.melanogaster and H.sapiens than to their counterparts in S.cerevisiae.Several splicing regulators,including SR proteins and CUGbinding proteins,were found in D.discoideum,but not in yeast.Our comprehensive catalog of spliceosomal proteins provides useful information for future studies of splicing in D.discoideum where the efficient genetic and biochemical manipulation will also further our general understanding of pre-mRNA splicing.

关 键 词：pre-mRNA splicing spliceosomal genes Dictyostelium discoideum comparative genomics splicing regulators 

分 类 号：O62[理学—有机化学]