Cryo-electron microscopy reconstructions of two types of wild rabbit hemorrhagic disease viruses characterized the structural features of Lagovirus  

作　　者：Zhongjun Hu Xiaojuan Tian Yujia Zhai Wei Xu Dong Zheng Fei Sun 

机构地区：[1]National Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [2]Department of Biochemistry and Molecular Biology,College of Life Sciences,Beijing Normal University,Beijing 100875,China

出　　处：《Protein & Cell》2010年第1期48-58,共11页蛋白质与细胞（英文版）

基　　金：This work was supported by National Natural Science Foundation of China(Grant Nos.30700029,30721003);Chinese Academy of Sciences(KGCX1-YW-13);the National Basic Research Program(973 Program)(Nos.2006CB806506,2006CB911001);the National Programs for High Technology Research and Development Program(863 Program)(No.2006AA02Z173).

摘　　要：Rabbit hemorrhagic disease was described in China in 1984 and can cause hemorrhagic necrosis of the liver within two or three days after infection.The etiological agent,rabbit hemorrhagic disease virus(RHDV),belongs to the Lagovirus genus in the Caliciviridae family.Compared to other calicivirus,such as rNV and SMSV,the structure of Lagovirus members is not well characterized.In this report,structures of two types of wild RHDV particles,the intact virion and the core-like particle(CLP),were reconstructed by cryo-electron microscopy at 11Åand 17Å,respectively.This is the first time the 3D structure of wild caliciviruses CLP has been provided,and the 3D structure of intact RHDV virion is the highest resolution structure in Lagovirus.Comparison of the intact virion and CLP structures clearly indicated that CLP was produced from the intact virion with the protrusion dissociated.In contrast with the crystal structures of recombinant Norovirus and San Miguel sea lion virus,the capsomers of RHDV virion exhibited unique structural features and assembly modes.Both P1 and P2 subdomains have interactions inside the AB capsomer,while only P2 subdomains have interaction inside CC capsomer.The pseudo atomic models of RHDV capsomers were constructed by homology modeling and density map fitting,and the rotation of RHDV VP60 P domain with respect to its S domain,compared with SMSV,was observed.Collectively,our cryo-electron microscopic studies of RHDV provide close insight into the structure of Lagovirus,which is important for functional analysis and better vaccine development in the future.

关 键 词：rabbit hemorrhagic disease virus CALICIVIRUS Lagovirus core-like particle cryo-electron microscopy reconstruction 

分 类 号：S85[农业科学—兽医学]