Crystal structure of the swine-origin A (H1N1)- 2009 influenza A virus hemagglutinin (HA) reveals similar antigenicity to that of the 1918 pandemic virus  

作　　者：Wei Zhang Jianxun Qi Yi Shi Qing Li Feng Gao Yeping Sun Xishan Lu Qiong Lu Christopher J.Vavricka Di Liu Jinghua Yan George F.Gao 

机构地区：[1]CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [2]Graduate University,Chinese Academy of Sciences,Beijing 100049,China [3]College of Life Science,University of Science and Technology of China,Hefei 230027,China [4]Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [5]College of Veterinary Medicine,China Agricultural University,Beijing 100094,China [6]Network Information Center,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [7]Beijing Institutes of Life Science,Chinese Academy of Sciences,Beijing 100101,China

出　　处：《Protein & Cell》2010年第5期459-467,共9页蛋白质与细胞（英文版）

基　　金：This work is supported by the intramural grant of the Chinese Academy of Sciences(Grant No.KSCX2-YW-R-158);the National Basic Research Program(973 Program)(Grant Nos.2010CB534004 and 2005CB523001);G.F.G.is a distinguished young investigator of the NSFC(Grant No.30525010);Dr.Christopher Vavricka is,partly,supported by the Fellowship for Young International Scientists of the Chinese Academy of Sciences(Grant No.2009Y2BS2).

摘　　要：Influenza virus is the causative agent of the seasonal and occasional pandemic flu.The current H1N1 influenza pandemic,announced by the WHO in June 2009,is highly contagious and responsible for global economic losses and fatalities.Although the H1N1 gene segments have three origins in terms of host species,the virus has been named swine-origin influenza virus(S-OIV)due to a predominant swine origin.2009 S-OIV has been shown to highly resemble the 1918 pandemic virus in many aspects.Hemagglutinin is responsible for the host range and receptor binding of the virus and is therefore a primary indicator for the potential of infection.Primary sequence analysis of the 2009 S-OIV hemagglutinin(HA)reveals its closest relationship to that of the 1918 pandemic influenza virus,however,analysis at the structural level is necessary to critically assess the functional significance.In this report,we report the crystal structure of soluble hemagglutinin H1(09H1)at 2.9Å,illustrating that the 09H1 is very similar to the 1918 pandemic HA(18H1)in overall structure and the structural modules,including the five defined antiboby(Ab)-binding epitopes.Our results provide an explanation as to why sera from the survivors of the 1918 pandemics can neutralize the 2009 S-OIV,and people born around the 1918 are resistant to the current pandemic,yet younger generations are more susceptible to the 2009 pandemic.

关 键 词：Influenza virus PANDEMIC 2009 1918 S-OIV hemagglutinin(HA) structure 

分 类 号：S85[农业科学—兽医学]