Structural basis for prokaryotic calciummediated regulation by a Streptomyces coelicolor calcium binding protein  

作　　者：Xiaoyan Zhao Hai Pang Shenglan Wang Weihong Zhou Keqian Yang Mark Bartlam 

机构地区：[1]Laboratory of Structural Biology,Tsinghua University,Beijing 100084,China [2]Center for Microbial Metabolism and Metabolic Engineering,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [3]Tianjin Key Laboratory of Protein Science,College of Life Sciences,Nankai University,Tianjin 300071,China

出　　处：《Protein & Cell》2010年第8期771-779,共9页蛋白质与细胞（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.30400259,30221003);the National Basic Research Program(973 Program)(Grant No.2007CB914301);the Tianjin Municipal Science and Technology Commission(Grant No.08SYSYTC00200).

摘　　要：The important and diverse regulatory roles of Ca2+in eukaryotes are conveyed by the EF-hand containing calmodulin superfamily.However,the calcium-regulatory proteins in prokaryotes are still poorly understood.In this study,we report the three-dimensional structure of the calcium-binding protein from Streptomyces coelicolor,named CabD,which shares low sequence homology with other known helix-loop-helix EF-hand proteins.The CabD structure should provide insights into the biological role of the prokaryotic calcium-binding proteins.The unusual structural features of CabD compared with prokaryotic EF-hand proteins and eukaryotic sarcoplasmic calcium-binding proteins,including the bending conformation of the first C-terminalα-helix,unpaired ligand-binding EF-hands and the lack of the extreme Cterminal loop region,suggest it may have a distinct and significant function in calcium-mediated bacterial physiological processes,and provide a structural basis for potential calcium-mediated regulatory roles in prokaryotes.

关 键 词：calcium-binding protein crystal structure Streptomyces coelicolor calcium-mediated regulation EFhand 

分 类 号：Q78[生物学—分子生物学]