Engineering of a genome-reduced host: practical application of synthetic biology in the overproduction of desired secondary metabolites  

作　　者：Hong Gao Ying Zhuo Elizabeth Ashforth Lixin Zhang 

机构地区：[1]CAS Key Laboratory of Pathogenic Microbiology&Immunology,Institute of Microbiology,Chinese Academy of Sciences(CAS),Beijing 100101,China [2]Graduate University of Chinese Academy of Sciences,Beijing 100049,China

出　　处：《Protein & Cell》2010年第7期621-626,共6页蛋白质与细胞（英文版）

基　　金：supported in part by grants from the National Programs for High Technology Research and Development Program(863 Program)(Grant Nos.2006AA09Z402,2007AA09Z443);the National Basic Research Program of China(973 Program)(Grant No.2004CB719601);National Natural Science Foundation of China(Grant Nos.30560001,30600001,30700015);National Key Technology R&D Program(2007BAI26B02);the National Science&Technology Pillar Program(No.200703295000-02);Important National Science&Technology Specific Projects(Nos.2008ZX09401-005,2009ZX09302-004);Science and Technology Planning Project of Guangdong Province,China(No.2006A50103001);Key Project of International Cooperation(2007DFB31620)。

摘　　要：Synthetic biology aims to design and build new biological systems with desirable properties,providing the foundation for the biosynthesis of secondary metabolites.The most prominent representation of synthetic biology has been used in microbial engineering by recombinant DNA technology.However,there are advantages of using a deleted host,and therefore an increasing number of biotechnology studies follow similar strategies to dissect cellular networks and construct genomereduced microbes.This review will give an overview of the strategies used for constructing and engineering reduced-genome factories by synthetic biology to improve production of secondary metabolites.

关 键 词：synthetic biology reduced-genome secondary metabolite 

分 类 号：F42[经济管理—产业经济]