基于网络药理学的山茱萸酒制科学内涵探究  

Exploration of Scientific Connotation of Processing of Shanzhuyu(Corni Fructus) with Wine Based on Network Pharmacology

作　　者：吴信华 瞿慧[1] 曹园[1] 方祝元[1] WU Xinhua;QU Hui;CAO Yuan;FANG Zhuyuan(Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,Jiangsu,China)

机构地区：[1]南京中医药大学附属医院,江苏南京210029

出　　处：《中华中医药学刊》2021年第5期81-85,I0020,I0021,共7页Chinese Archives of Traditional Chinese Medicine

基　　金：国家自然科学基金(81102772);江苏省中医药局科技项目(JD201813)。

摘　　要：目的利用网络药理学方法探讨山茱萸酒制前后功效变化的分子机制,从而揭示山茱萸酒制的科学内涵。方法查阅文献收集山茱萸酒制前后的差异成分,联合SIB、SEA、STITCH、TCMSP多个数据库分析所有成分的作用靶点,利用STRING数据库和Metascape工具筛选出核心靶点。运用Cytoscape 3.2.1软件构建差异成分-靶点网络、蛋白相互相互作用(PPI)网络,通过DAVID进行基因本体(GO)功能富集分析和基于京都基因与基因组百科全书(KEGG)通路富集分析,最后运用BioGps数据库对所得靶点进行器官定位。结果共筛选出9种差异成分和46个相应靶点,通过GO功能分析和KEGG通路分析,共得到85个GO条目和14条KEGG通路,器官定位结果表明作用的核心病位为肝。结论分析结果表明,山茱萸酒制后的功效改变是化学成分变化及其相互作用的结果。酒萸肉主要通过cAMP、神经活性配体-受体相互作用、PI3K-Akt等信号通路发挥补益肝肾、免疫调节和抗衰老作用。Objective Processing with wine is one of the important processing methods of Shanzhuyu(Corni Fructus), which may significantly enhance its effects of nourishing liver and kidney, as well as preventing decrepitude and strengthening immunity. This study aimed to clarify the molecular mechanism of how the ingredient variation affected the efficacy of processed Shanzhuyu(Corni Fructus), and elucidate the scientific connotation of this processing methodology based on network pharmacology. Methods The major different ingredients between crude and processed Shanzhuyu(Corni Fructus) were screened out based on literature. The potential targets of these ingredients were predicted according to SIB, SEA, STITCH, TCMSP database, and the core targets were selected out by STRING database and Metascape. Cytoscape software were used to construct protein-protein interactions network and ingredient-target network. GO function and KEGG pathways involved in the targets were analyzed by DAVID database. BioGps database was used to locate the target organs. Results Nine differential components and 43 core targets were screened out. The network analysis results showed that 85 GO terms and 14 KEGG pathways were involved. The results of organ localization showed that the core disease site was liver. Conclusion The changes in the efficacy between crude and processed Shanzhuyu(Corni Fructus) resulted from chemical composition alteration and their interactions. The enhancement of pharmaceutical efficacy of processed Shanzhuyu(Corni Fructus) may be accomplished by regulation of neuroactive ligand-receptor interaction, cAMP signaling pathway, PI3 K-Akt signaling pathway and so on.

关 键 词：山茱萸 酒制 网络药理学 差异成分 靶点 功效 

分 类 号：R283[医药卫生—中药学]