槐定碱对永久性大脑中动脉栓塞大鼠脑细胞的作用及机制研究  

Mechanism of sophoridine on cerebral cell in rats with permanent middle cerebral artery occlusion

作　　者：薛玉梅 李献军 孟岩 宋哲[1] 刘惠钦 李晓兵 XUE Yu-mei;LI Xian-jun;MENG Yan;SONG Zhe;LIU Hui-qin;LI Xiao-bing(Department of Neurology,Shijiazhuang People’s Hospital,Shijiazhuang 050027,China;Department of Pharmacy,Shijiazhuang People’s Hospital,Shijiazhuang 050027,China)

机构地区：[1]石家庄市人民医院神经内科,河北石家庄050027 [2]石家庄市人民医院药学部,河北石家庄050027

出　　处：《中草药》2021年第11期3306-3311,共6页Chinese Traditional and Herbal Drugs

基　　金：石家庄市科学技术研究与发展指导计划项目(161460713)。

摘　　要：目的探究槐定碱对永久性大脑中动脉栓塞(permanent middle cerebral artery occlusion,p MCAO)大鼠脑细胞的作用及机制。方法 SD大鼠随机分为对照组、模型组和槐定碱低、中、高剂量(2.5、5.0、10.0mg/kg)组,给予槐定碱5d后,建立p MCAO大鼠模型。考察槐定碱对pMCAO大鼠神经功能评分的影响;考察槐定碱对pMCAO大鼠脑梗死率的影响;采用流式细胞术检测各组大鼠缺血半暗带区细胞凋亡率;采用苏木素-伊红(HE)染色法观察各组大鼠脑组织病理变化;采用Western blotting法考察各组大鼠脑组织B细胞淋巴瘤-2(B-cell lymphoma 2,Bcl-2)、Bcl-2相关X蛋白(Bcl-2 associated Xprotein,Bax)和半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)蛋白表达情况。结果与模型组比较,槐定碱组大鼠神经缺陷症状改善,脑梗死率显著降低(P<0.05、0.01),缺血半暗带细胞凋亡率明显降低(P<0.05、0.01),脑组织损伤有所改善,脑组织Caspase-3和Bax蛋白表达水平显著降低(P<0.05、0.01),Bcl-2蛋白表达水平显著升高(P<0.05、0.01)。结论槐定碱对pMCAO大鼠脑细胞具有保护作用,其机制可能与上调Bcl-2及下调Bax、Caspase-3蛋白表达水平,进而抑制细胞凋亡有关。Objective To explore the effect and mechanism of sophoridine on brain cells in rats with permanent middle cerebral artery occlusion(pMCAO). Methods SD rats were randomly divided into control group,model group,low-,medium-and highdose(2.5,5.0,10.0 mg/kg) sophoridine groups. After ip sophoridine for 5 d,pMCAO rat model was established. Effect of sophoridine on neurological function score in pMCAO rats were investigated;Effect of sophoridine on cerebral infarction rate in pMCAO rats were investigated;Flow cytometry was used to detect the cell apoptosis rate in ischemic penumbra of rats in each group;Hematoxylin-eosin(HE) staining method was used to observe the pathological changes in brain tissue of rats in each group;Western blotting was used to investigate expressions of B-cell lymphoma-2(Bcl-2),Bcl-2 associated X protein(Bax),and Caspase-3 in brain tissue of rats. Results Compared with model group,neurological deficit symptoms of rats in sophoridine group were improved,cerebral infarction rate was significantly reduced(P < 0.05,0.01),cell apoptosis rate in ischemic penumbra was significantly reduced(P < 0.05,0.01),tissue damage was improved,expressions of Caspase-3 and Bax in brain tissue were significantly reduced(P < 0.05,0.01),and Bcl-2 expression was significantly increased(P < 0.05,0.01). Conclusion Sophoridine has a protective effect on brain cells in pMCAO rats,its mechanism may be related to up-regulation of Bcl-2 and down-regulation of Bax and Caspase-3 expressions,thereby inhibiting cell apoptosis.

关 键 词：槐定碱 永久性大脑中动脉栓塞 脑细胞凋亡 BCL-2 BAX CASPASE-3 

分 类 号：R285.5[医药卫生—中药学]