一测多评法同时测定阿娜尔妇洁液中7种成分  

Simultaneous determination of seven constituents in A’naer Vigills by QAMS

作　　者：杨莎[1] 宋忠兴[1] 唐志书[1] 史鑫波 刘红波[1] 马虎强 解亚娟 王昌利[1,2] YANG Sha;SONG Zhong-xing;TANG Zhi-shu;SHI Xin-bo;LIU Hong-bo;MA Hu-qiang;XIE Ya-juan;WANG Chang-li(Shaanxi Collaborative Innovation Center of Chinese Medicine Resources Industrialization of Shaanxi University of Chinese Medicine,Shaanxi Key Laboratory of Research&Development of Characteristic Qin Medicine Resources(Cultivation),Shaanxi Institute of TCM Industry,Xianyang 712083,China;Shaanxi Haitian Pharmaceutical Co.,Ltd.,Xianyang 712083,China)

机构地区：[1]陕西中医药大学/陕西省中药资源产业化省部共建协同创新中心/秦药特色资源研究开发重点实验室(培育)/陕西中药产业研究院,陕西咸阳712083 [2]陕西海天制药有限公司,陕西咸阳712083

出　　处：《中草药》2021年第12期3560-3567,共8页Chinese Traditional and Herbal Drugs

基　　金：中央本级重大增减支项目“名贵中药资源可持续利用能力建设项目”(2060302);国家现代中药材产业技术体系咸阳综合试验站补贴项目(2019JCW-06);中药大品种品牌价值提升示范研究(202190025)。

摘　　要：目的建立阿娜尔妇洁液中7种成分花椒毒酚、花椒毒素、异茴芹内酯、羟基-α-山椒素、羟基-β-山椒素、欧前胡素、蛇床子素的一测多评法,验证该方法在阿娜尔妇洁液质量分析中应用的科学性与可行性。方法采用HPLC法,以花椒毒酚为内参物,建立花椒毒素、异茴芹内酯、羟基-α-山椒素、羟基-β-山椒素、欧前胡素、蛇床子素的相对校正因子(fs/i),并利用fs/i计算阿娜尔妇洁液中该7种成分的含量,同时采用外标法计算各成分的含量,并比较2种方法差异。结果花椒毒素、异茴芹内酯、羟基-α-山椒素、羟基-β-山椒素、欧前胡素、蛇床子素的fs/i分别为1.0057、1.2721、2.4210、3.1606、1.2054、1.2993。一测多评法与外标法得到的10批阿娜尔妇洁液样品含量测定结果RSD<2.0%,没有显著差异。结论在对照品短缺的情况下,以花椒毒酚为内参物,建立的一测多评法可用于阿娜尔妇洁液中多指标成分的含量测定和质量评价。Objective To establish a quantitative analysis of multi-components by single marker(QAMS)method for the simultaneous determination of seven components of xanthotol,xanthotoxin,isopimpinellin,hydroxy-α-sandolin,hydroxy-β-sansolin,imperatorin and osthol in A’naer Vigills(阿娜尔妇洁液).Further to verify the scientificity and feasibility of the method in the quality analysis in A’naer Vigills.Methods The relative factors(fs/i)of xanthotoxin,isopimpinellin,hydroxy-α-sandolin,hydroxy-β-sansolin,osthol and imperatorin were established by HPLC method with xanthotol as internal reference,which was used to calculate the content of seven constituents.The difference between QAMS and external standard method(ESM)was compared to evaluate the accuracy of QAMS.Results The fs/i of xanthotoxin,isopimpinellin,hydroxy-α-sandolin,hydroxy-β-sansolin,imperatorin and osthol were 1.0057,1.2721,2.4210,3.1606,1.2054 and 1.2993,respectively.The content determination results of ten batches of A’naer Vigills were calculated and compared between the methods of QAMS and ESM,and no significant difference was observed with RSD<2.0%.Conclusion In the case of shortage of reference substance,the established QAMS method can be used for the content determination and quality evaluation of the multi-index components in A’naer Vigills with xanthotol as internal reference.

关 键 词：阿娜尔妇洁液 一测多评 花椒毒酚 花椒毒素 异茴芹内酯 羟基-α-山椒素 羟基-β-山椒素 欧前胡素 蛇床子素 

分 类 号：R286.02[医药卫生—中药学]