聚磷酸酯/聚乙二醇复合水凝胶的构建及性能  

Synthesis and characterization of polyphosphoester/polyethylene glycol composite hydrogels

作　　者：张正海 杨显珠 Zhenghai Zhang;Xianzhu Yang(School of Medicine,South China University of Technology,Guangzhou 510006,China)

机构地区：[1]华南理工大学医学院,广州510006

出　　处：《科学通报》2021年第18期2312-2318,共7页Chinese Science Bulletin

基　　金：国家自然科学基金(51822302)资助。

摘　　要：在水溶性大分子聚磷酸酯二丙烯酸酯(PEEP-DA)与聚乙二醇二丙烯酸酯(PEG-DA)中加入光引发剂I2959,在紫外光照射下交联形成三维水凝胶体系.研究结果表明,与聚乙二醇水凝胶相比,聚磷酸酯/聚乙二醇复合水凝胶具有更好的生物可降解性.其中,PEEP-DA/PEG-DA比例为5:25的复合水凝胶植入到小鼠体内28 d后基本完全消失.而包裹骨髓间充质干细胞(BMSCs)后,复合水凝胶体系中细胞活性更好;聚磷酸酯/聚乙二醇复合水凝胶能够显著增强碱性磷酸酶活性,提高钙沉积.本研究提供了一种新的聚磷酸酯/聚乙二醇复合水凝胶体系,在作为细胞支架方面有一定应用潜力.The hydrogel,which has a three-dimensional(3 D)cross-linked hydrophilic network structure,is widely used as biomedical scaffold for local drug delivery and tissue engineering.Especially,with the continuous development of 3 D printing technology,hydrogel scaffolds are increasingly used in the field of regenerative medicine.Polyphosphoester is a class of biodegradable polymers with repeated phosphoester linkages in the backbone,and a series of polyphosphoester materials have been synthesized for biomedical applications.For instance,a variety of amphiphilic polyphosphoester-based copolymers have been synthesized by Wang’s group,and used as the nanocarriers of hydrophobic anticancer drugs as well as si RNA.In addition,Yan’s group has prepared hyper-branched polyphosphoester as the drug delivery systems of hydrophobic anticancer drugs and photodynamic agents.Recently,Wooley and co-workers have synthesize the amphiphilic co-polymer of poly(ethylene oxide)and polyphosphoester,and used to encapsulated paclitaxel(PTX)with high contents for cancer therapy.And,they also conjugated the PTX into the side chain of polyphosphoester with p H-labile linkages for efficient tumor treatments.As the tissue engineering scaffold,polyphosphoester-based scaffolds were first used for fabrication of nerve guide conduits.And then,Elisseeff and co-workers have found the great potentials of phosphate-containing hydrogel for bone tissue engineering.In addition,Leong et al.found that the photocrosslinkable polyphosphoester hydrogel exhibited good compatibility and potentially osteoinductive ability for goat bone marrow-derived mesenchymal stem cells.Therefore,polyphosphoester hydrogel could be a promising scaffold for bone tissue engineering.In this study,water-soluble macromers polyphosphate diacrylate(PEEP-DA)and polyethylene glycol diacrylate(PEGDA)were first synthesized,and characterized by1 H NMR.And then,the PEEP/PEG composite hydrogels at various polyphosphoester contents were controlled by varying the feed ratio of the macromers PEE

关 键 词：聚磷酸酯 水凝胶 支架材料 生物可降解支架 

分 类 号：R318.08[医药卫生—生物医学工程]