System approaches reveal the molecular networks involved in neural stem cell differentiation  

作　　者：Kai Wang Haifeng Wang Jiao Wang Yuqiong Xie Jun Chen Huang Yan Zengrong Liu Tieqiao Wen 

机构地区：[1]Laboratory of Molecular Neurobiology,School of Life Sciences,Shanghai University,Shanghai 200444,China [2]School of Communication and Information Engineering,Shanghai University,Shanghai 200444,China [3]College of Life Science,Wuhan University,Wuhan 430072,China [4]College of Chemical Engineering and Technology,Wuhan University of Science and Technology,Wuhan 430081,China [5]Institute of Systems Biology,Shanghai University,Shanghai 200444,China

出　　处：《Protein & Cell》2012年第3期213-224,共12页蛋白质与细胞（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.31070954,11172158 and 10832006);the Shanghai Commission of Science and Technology Basic Research Fund(No.09JC1406600);the Shanghai Education Committee and Shanghai Leading Academic Discipline Project(J50103,key discipline"Molecular Physiology").

摘　　要：The self-renewal and multipotent potentials in neural stem cells(NSCs)maintain the normal physiological functions of central nervous system(CNS).The ab-normal differentiation of NSCs would lead to CNS disorders.However,the mechanisms of how NSCs differentiate into astrocytes,oligodendrocytes(OLs)and neurons are still unclear,which is mainly due to the complexity of differentiation processes and the limita-tion of the cell separation method.In this study,we modeled the dynamics of neural cell interactions in a systemic approach by mining the high-throughput genomic and proteomic data,and identified 8615 genes that are involved in various biological processes and functions with significant changes during the differentiation processes.A total of 1559 genes are specifically expressed in neural cells,in which 242 genes are NSC specific,215 are astrocyte specific,551 are OL specific,and 563 are neuron specific.In addition,we proposed 57 transcriptional regulators specifically expressed in NSCs may play essential roles in the development courses.These findings provide more comprehensive analysis for better understanding the endogenous mechanisms of NSC fate determination.

关 键 词：neural stem cell cell differentiation mo-lecular networks 

分 类 号：R32[医药卫生—人体解剖和组织胚胎学]