The enzymatic activity of Arabidopsis protein arginine methyltransferase 10 is essential for flowering time regulation  

作　　者：Lifang Niu Falong Lu Taolan Zhao Chunyan Liu Xiaofeng Cao 

机构地区：[1]State Key Laboratory of Plant Genomics and National Center for Plant Gene Research,Institute of Genetics and Developmental Biology,Chinese Academy of Sciences,Beijing 100101,China [2]Institute for Agricultural Biosciences,Oklahoma State University,Ardmore,OK 73401,USA [3]Lineberger Comprehensive Cancer Center,University of North Carolina at Chapel Hill,Chapel Hill,NC 27599,USA [4]Graduate School of the Chinese Academy of Sciences,Beijing 100039,China

出　　处：《Protein & Cell》2012年第6期450-459,共10页蛋白质与细胞（英文版）

基　　金：supported by the National Basic Research Program of China(Nos.2011CB915400 and 2009CB941500 to X.C.);the National Natural Science Foundation of China(Grant Nos.30930048 and 30921061 to X.C.,and 90919033 to C.L.);the Chinese Academy of Sciences(No.KSCX2-EW-Q-24-02 to C.L.)。

摘　　要：Arabidopsis AtPRMT10 is a plant-specific type I protein arginine methyltransferase that can asymmetrically dimethylate arginine 3 of histone H4 with auto-methylation activity.Mutations of AtPRMT10 derepress FLOWERING LOCUS C(FLC)expression resulting in a late-flowering phenotype.Here,to further investigate the biochemical characteristics of AtPRMT10,we analyzed a series of mutated forms of the AtPRMT10 protein.We demon-strate that the conserved“VLD”residues and“double-E loop”are essential for enzymatic activity of AtPRMT10.In addition,we show that Arg54 and Cys259 of AtPRMT10,two residues unreported in animals,are also important for its enzymatic activity.We find that Arg13 of AtPRMT10 is the auto-methylation site.However,substitution of Arg13 to Lys13 does not affect its enzymatic activity.In vivo complementation assays reveal that plants expressing AtPRMT10 with VLD-AAA,E143Q or E152Q mutations retain high levels of FLC expression and fail to rescue the late-flowering phenotype of atprmt10 plants.Taken together,we conclude that the methyltransferase activity of AtPRMT10 is essential for repressing FLC expression and promoting flowering in Arabidopsis.

关 键 词：protein arginine methyltransferases(PRMTs) FLOWERING methyltransferase activity 

分 类 号：Q94[生物学—植物学]