基于微根管技术的盐胁迫下小麦根系生长原位监测方法  

In-situ Monitoring Method of Wheat Root Growth under Salt Stress Using Minirhizotron Technique

作　　者：李燕丽[1,2] 王昌昆 卢碧林[2] 李继福[2] 潘贤章[1] LI Yanli;WANG Changkun;LU Bilin;LI Jifu;PAN Xianzhang(State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Nanjing 210008,China;College of Agriculture,Yangtze University,Jingzhou,Hubei 434025,China)

机构地区：[1]土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京210008 [2]长江大学农学院,湖北荆州434025

出　　处：《土壤学报》2021年第3期599-609,共11页Acta Pedologica Sinica

基　　金：国家重点研发计划项目(2018YFC1800104-02,2018YFD0301301);中国农业科学院农田灌溉研究所开放课题(FIRI2018-07-02)资助。

摘　　要：常用的作物根系生长监测一般采用破坏性采样方法,如土钻法和挖掘法等,虽然精度较高,但很难实现对作物根系生长的原位重复观测。采用桶栽法,利用微根管技术对分蘖期、返青期、拔节期和孕穗期的小麦根系进行了连续观测和采样,获取不同盐胁迫下小麦根长密度和根长等参数,研究不同生长时期小麦根系生长参数随土壤深度分布的规律。结果显示,微根管法获得的小麦根长密度与土钻法所得到的结果呈极显著正相关(r=0.91),且在拔节期和孕穗期二者的相关性最好。通过不同时期根系图像对比及根系参数分析发现,小麦根系在0~10 cm土层分布最多,并随深度增加而减少。此外,随着土壤盐含量的增加,各生长期根长变短;在分蘖期,土壤盐分含量最高(S5,盐分含量6.61 g·kg^(-1))的小麦根系长度不足对照处理的1/2,至孕穗期,其根系长度甚至低至对照处理的1/3,说明小麦根系受盐分胁迫影响较大,且以孕穗期受胁迫程度最严重,尤其当土壤盐含量超过3 g·kg^(-1)时影响最明显。由此可见,与传统破坏性取样方法相比,微根管技术结合图像处理技术可更好地快速、无损获取小麦根系生长的相关参数,为盐渍化区域作物根系的原位观测研究提供了新的方法。【Objective】Generally,root is the main organ of a plant to absorb water and nutrients in soil,and how it grows and distributes is an important indicator of a crop in growth and development.The commonly used methods for monitoring crop root growth,such as the soil drilling and excavation methods,are what we call destructive sampling methods.Although they are usually quite high in accuracy,they are not suitable for in situ monitoring of root growth.Therefore,the minirhizotron technique is recommended as an effective method for in-situ monitoring of crop root growth thanks to its rapid and non-destructive approach.【Method】 In order to reveal impacts of salt stress on root growth of wheat, a pot culture experiment was conducted, with soil salt content varying along a gradient from 0.61(CK), 1.61(S1), 2.61(S2), 3.61(S3), 4.61 (S4) to 5.61 g·kg^(-1) (S5). And the minirhizotron technique was used to- collect digital images of the roots at tillering, reviving, jointing and booting growing stages. Then root length, root length density and their distribution in the soil as affected by salt stress were determined with the aid of the digital image processing technology. 【Result】 Results show that with the aid of the minirhizotron technique, growth and development progresses of the wheat root in the experiment could be intuitively monitored. In the experiment the root length density obtained with the technique was found to be significantly and positively related with that with the soil drilling method (r =0.91), especially at the jointing and booting stages of the crop. Moreover, comparisons of the root images with the root parameters analysis relative to growing stage of the crop revealed that wheat root distributed mostly in the 0-10 cm soil layer and decreased with soil depth, and that root length decreased with rising soil salt content at all growing stages. For example, at the tillering stage, the root length in treatment S5 (S5, 6.61 g·kg^(-1)) was less than half of that in CK, and at the booting stage it w

关 键 词：微根管技术 盐胁迫 根长密度 根长 小麦 图像处理技术 

分 类 号：S127[农业科学—农业基础科学] S151.9