马兜铃酸和缺血再灌注所致急性肾损伤后慢性 肾脏病小鼠模型的比较  

Comparison of mouse models of chronic kidney disease after acute kidney injury caused by aristolochic acid and ischemia-reperfusion

作　　者：谭微 邓军辉 吴志芬 郑卢权 杨聚荣 TAN Wei;DENG Junhui;WU Zhifen;ZHENG Luquan;YANG Jurong(Department of Nephrology,Third Affiliated Hospital of Chongqing Medical University,Chongqing 401120,China)

机构地区：[1]重庆医科大学附属第三医院肾内科,401120

出　　处：《重庆医学》2021年第13期2182-2186,2193,共6页Chongqing medicine

基　　金：重庆市教委科学技术研究项目(KJQN202000439);重庆医科大学附属第三医院科研孵化项目(KY08029)。

摘　　要：目的观察马兜铃酸和缺血再灌注所致急性肾损伤(AKI)后慢性肾脏病(CKD)动物模型的差异。方法BALB/c小鼠分为马兜铃酸损伤(AAI)组、AAI对照组、缺血再灌注损伤(IRI)组、IRI对照组,AAI组以5 mg/kg剂量腹腔内注射马兜铃酸,AAI对照组腹腔注射等量生理盐水;IRI组予37℃双侧肾动脉夹闭缺血32 min,IRI对照组予假手术;于给药或再灌注后1、3、7、14、28、42 d收集标本。观察不同时间点各组小鼠的死亡情况及体重,自动分析法检测各小鼠血清肌酐、血清尿素氮水平,肾组织PAS染色评估肾损伤程度,Masson染色评估肾脏纤维化情况。结果IRI组死亡率显著高于AAI组(P<0.05);AAI组小鼠体重下降更显著(P<0.05);IRI和AAI组小鼠血肌酐、尿素水平均高于对照组(P<0.05);IRI组术后第1天肾损伤最重,AAI组第7天肾损伤最重,至造模后42 d部分肾小管仍无完整结构;与对照组比较,造模后14 d IRI和AAI组均开始出现纤维化,AAI组纤维化阳性面积多于IRI组(P<0.05)。结论AAI和IRI均可成功构建小鼠AKI-CKD模型,但AAI模型更简单、稳定。Objective To observe the differences in chronic kidney disease(CKD)animal models after acute kidney injury(AKI)caused by aristolochic acid injury(AAI)and ischemia-reperfusion injury(IRI).Methods BALB/c mice were divided into the AAI group,AAI control group,IRI group and IRI control group,the mice in the AAI group were intraperitoneally injected with 5 mg/kg aristolochic acid,and the AAI control group was replaced with the same amount of normal saline;the mice in the IRI group were given 37℃bilateral renal artery clipping ischemia for 32 min,and the IRI control group was given the sham operation;the specimens were collected on 1,3,7,14,28,42 d after administration or reperfusion.The death and weight of each group of mice at different time points were observed,the levels of serum creatinine and serum urea nitrogen in mice were measured by the automatic analysis method,and the renal injury degrees were evaluated by the renal tissue PAS staining,and the renal fibrosis was evaluated by the Masson staining.Results The mortality rate of the IRI group was significantly higher than that of the AAI group(P<0.05);the weight loss of the AAI group was more significant(P<0.05);the serum creatinine and urea levels in the IRI and AAI groups were higher than those in the control group(P<0.05);the IRI group had the heaviest kidney damage on postoperative 1 d,while the AAI group had the heaviest kidney damage on postoperative 7 d,the partial renal tubules had no complete structure until 42 d after the modeling;compared with the control group,the fibrosis appeared in the IRI and AAI groups on 14 d after the establishment of the model.The positive area of fibrosis in the AAI group was more than that in the IRI group(P<0.05).Conclusion Both AAI and IRI can successfully construct the mouse AKI-CKD model,but the AAI model is simpler and more stable.

关 键 词：马兜铃酸 缺血再灌注 急性肾损伤 慢性肾脏病 动物模型 

分 类 号：R941.427[医药卫生—药剂学]