石甘散联合托吡酯对癫痫幼鼠认知功能及海马组织CB2R、MDA、SOD水平的影响  

Effect of Shigan Powder combined with topiramate on cognitive function and levels of CB2R,MDA and SOD in hippocampus of epileptic young rats

作　　者：杨昕憓 于滨[1] YANG Xinhui;YU Bin(Department of Neurology,First Affiliated Hospital of Harbin Medical University,Harbin,Heilongjiang 150000,China)

机构地区：[1]哈尔滨医科大学附属第一医院神经内科,150000

出　　处：《重庆医学》2021年第13期2194-2197,共4页Chongqing medicine

摘　　要：目的探讨石甘散联合托吡酯对癫痫幼鼠认知功能及海马组织大麻素受体2(CB2R)、丙二醛(MDA)、超氧化物歧化酶(SOD)水平的影响。方法将60只SPF级SD幼鼠分为对照组、模型组、石甘散组、托吡酯组、联合组,每组12只。对照组腹腔注射1 mL的0.9%氯化钠溶液,连续注射28 d。其余4组均于每日固定时间腹腔注射戊四氮溶液,建模成功后,对照组及模型组给予0.9%氯化钠溶液(10 mg/kg)灌胃,每日2次。石甘散组给予1 g/kg石甘散灌胃,每日1次,托吡酯组给予18 mg/kg托吡酯灌胃,每日1次,联合组给予1 g/kg石甘散和18 mg/kg托吡酯灌胃,每日1次。连续干预28 d后进行Morris水迷宫测试。测试结束后立即处死幼鼠,采用考马斯亮蓝法检测海马组织超氧化物歧化酶(SOD)及丙二醛(MDA)水平,采用Western blot法检测海马组织大麻素2型受体水平。结果水迷宫结果显示,5组在同一时间点找到平台时间由高到低分别为模型组、石甘散组、托吡酯组、联合组、对照组,穿越原平台位置次数及在平台象限搜索时间所占百分比由低到高分别为模型组、石甘散组、托吡酯组、联合组、对照组。各组CB2R及MDA水平分别为模型组[(0.39±0.09)vs.(12.98±2.87)nmol/mg]、联合组[(0.30±0.06)vs.(9.88±1.97)nmol/mg]、石甘散组[(0.20±0.05)vs.(7.32±1.44)nmol/mg]、托吡酯组[(0.19±0.05)vs.(7.56±1.32)nmol/mg]、对照组[(0.13±0.04)vs.(5.19±1.12)nmol/mg],而SOD水平由低到高分别为模型组、石甘散组、托吡酯组、联合组、对照组。结论石甘散联合托吡酯可显著改善癫痫幼鼠认知功能,并提高海马组织SOD活性及CB2R水平,降低MOD含量,抑制氧化反应,减轻自由基对脑组织的损伤。Objective To investigate the effect of Shigan Powder combined with topiramate on the cognitive function and the levels of CB2R,MDA and SOD in hippocampus of epileptic young rats.Methods Sixty SPF-grade SD rats were randomly divided into the control group,model group,Shigan Powder group,topiramate group and combination group,12 cases in each group.The control group was intraperitoneally injected by 1 mL of 0.9%sodium chloride solution for consecutive 28 d,and the remaining four groups were intraperitoneally injected with pentylenetetrazol solution at fixed time every day.After successful modeling,the control group and model group were given 0.9%sodium chloride solution(10 mg/kg)by gavage,twice daily,the Shigan Powder group was given the gavage of 1.0 g/kg Shigan Powder,the topiramate group was given the gavage of 18 mg/kg topiramate,once daily,and the combination group was given the gavage of 1.0 g/kg Shigan Powder and 18 mg/kg topiramate,once daily.After 28 d continuous intervention,the Morris water maze test was performed.Immediately after the test,the young rats were sacrificed.The levels of superoxide dismutase(SOD)and malondialdehyde(MDA)in the hippocampus were measured by Coomassie Brilliant Blue.The levels of cannabinoid type 2 receptors in the hippocampus were detected by Western blot.Results The water maze results showed that the time of five groups finding the platform at the same time point from high to low was the model group>Shigan Powder group>topiramate group>combination group>control group,the number of times to cross the original platform position and the percentage of the search time in the platform quadrant from low to high was the model group<Shigan Pwoder group<topiramate group<combination group<control group.The levels of CB2R and MDA in each group were(0.39±0.09)nmol/mg and(12.98±2.87)nmol/mg in the model group,(0.30±0.06)nmol/mg and(9.88±1.97)nmol/mg in the combination group,(0.20±0.05)nmol/mg and(7.32±1.44)nmol/mg in the Shigan Powder group,(0.19±0.05)nmol/mg and(7.56±1.32)nmol/mg

关 键 词：癫痫 海马 认知功能 石甘散 托吡酯 

分 类 号：R971.6[医药卫生—药品]