虎潜丸对去卵巢骨质疏松症大鼠骨组织的保护作用及其机制研究  

Study on the Protective Effect and Mechanism of Huqian Pill on Bone Tissue of Ovariectomized Osteoporosis Rats

作　　者：王进[1] 张倩[1,2] 唐好知 杨旭 王花欣[1,2] WANG Jin;ZHANG Qian;TANG Haozhi;YANG Xu;WANG Huaxin(Shandong University of Traditional Chinese Medicine,Ji′nan 250355,China;Shandong Co-Innovation Center of Classic Traditional Chinese Medicine Formula,Ji′nan 250355,China)

机构地区：[1]山东中医药大学,济南250355 [2]山东省中医经典名方协同创新中心,济南250355

出　　处：《世界中医药》2021年第12期1829-1833,1838,共6页World Chinese Medicine

基　　金：国家自然科学基金委员会青年科学基金项目(82004087);山东省中医药科技发展计划项目(2017-004);山东省高等学校科技计划项目(J18KA271);山东省自然科学基金重大基础研究项目(ZR2019ZD23);国家级大学生创新训练计划项目(201910441010);山东中医药大学青年创新团队项目。

摘　　要：目的:探讨虎潜丸对去卵巢骨质疏松症大鼠骨组织的保护作用,以及通过调控碱性磷酸酶(ALP)、白细胞介素-6(IL-6)和血清骨保护素(OPG)的表达而防治骨质疏松症的机制研究。方法:采用卵巢切除法复制绝经后骨质疏松症大鼠模型,随机分为模型组、虎潜丸组、雌二醇组,同时设置假手术组作为对照,各组分别给予相应的药物灌胃。灌胃12周后取血处死,称量各组大鼠的体质量、子宫重量;取大鼠左侧股骨、胫骨进行骨密度检测;全自动生化分析仪检测各组大鼠血清中血糖、血脂、钙磷的变化;ELISA检测各组大鼠血清中IL-6、ALP、OPG的水平。结果:与假手术组比较,模型组大鼠骨密度显著下降(P<0.05),骨微结构破坏明显;与模型组比较,虎潜丸组大鼠骨密度增加,骨小梁的完整性升高,骨微结构得到改善,体质量增长明显下降,血脂血糖水平降低,IL-6、ALP含量均有所降低,OPG含量升高,血钙含量有明显上升,差异有统计学意义(P<0.05)。结论:虎潜丸能够提高去卵巢大鼠的骨量,防治绝经后骨质疏松症,其作用机制可能与降低ALP、IL-6,提高OPG含量有关。Objective:To explore the protective effect of Huqian Pill on bone tissue of ovariectomized osteoporosis rats and the mechanism of prevention and treatment of osteoporosis by regulating the expression of ALP,IL-6 and OPG.Methods:The model of postmenopausal osteoporosis rats was made by ovariectomy.The rats were randomly divided into a model group,a Huqian Pills group and an estradiol group.At the same time,a sham operation group was set up as a control,and each group was given corresponding drugs by gavage.After 12 weeks of gavage,blood was taken and executed,the body mass and uterus mass of each group were weighed;the bone density of left femur and tibia of each group were measured;the changes of blood glucose,blood lipid and calcium phosphorus in serum of each group were detected by automatic biochemical analyzer;the contents of IL-6,ALP and OPG in rat serum of each group were detected by ELISA.Results:Compared with sham group,model group BMD decreased significantly(P<0.05);there was significant destruction of bone microarchitecture;compared with model group,Huqian Pill increased bone density of rats,increases the integrity of trabecular bone,bone microarchitecture was improved,the amount of weight gain decreased,blood lipid and glucose levels decreased,IL-6,and ALP content were decreased,levels of OPG elevated,calcium content significantly increased.The differences were statistically significant(P<0.05).Conclusion:Haqian Pills can improve bone mass in ovariectomized rats,prevent postmenopausal osteoporosis,and its mechanism may reduce ALP,IL-6,and improve OPG related content.

关 键 词：虎潜丸 绝经后骨质疏松症 骨密度 去卵巢大鼠 雌激素 白细胞介素-6 中医药治疗 

分 类 号：R285.5[医药卫生—中药学] R274.9[医药卫生—中医学]