防控烟草青枯病植物免疫诱抗剂的筛选及其防治效果测定  

Screening of Plant Immune Inducer Against Tobacco Bacterial Wilt and Determination of Its Control Effect

作　　者：薛梅 曾婉琳 张荣[1,2] 冯淑杰[1,2] XUE Mei;ZENG Wanlin;ZHANG Rong;FENG Shujie(College of Horticultrue,South China Agricultural University,Guangzhou 510642,China;Guangdong Integrative Microbiology Research Centre,South China Agricultural University,Guangzhou 510642,China)

机构地区：[1]华南农业大学园艺学院,广东广州510642 [2]华南农业大学广东省群体微生物研究中心,广东广州510642

出　　处：《广东农业科学》2021年第6期87-92,共6页Guangdong Agricultural Sciences

基　　金：国家重点研发计划项目(2018YFD0201102-4)。

摘　　要：【目的】为了探索可用于烟草青枯病防治的植物免疫诱抗剂,对4种药剂的抑菌活性和防治效果进行筛选和测定。【方法】利用平板抑菌和盆栽防控效果测定分析,以及测定其最小抑菌浓度(MIC)值和最小杀菌浓度(MBC)值研究不同植物免疫诱抗剂对烟草青枯病的防治效果。【结果】氨基寡糖素和纳米NA NO ZnO显示了一定的平板抑菌效果,其中5000μg/mL的氨基寡糖素平板抑菌效果最佳,抑菌圈直径达35.2 mm,其MIC和MBC分别为1000、2000μg/mL;不同浓度智能聪和阿泰灵均没有显示平板抑菌效果;而盆栽防治效果显示,智能聪对青枯病的防治效果最好,接种后10 d进入发病高峰期,此时其防效可达57%,显著高于同一时期链霉素处理37%的防治效果,且可延缓病害发生;而平板抑菌效果显著的氨基寡糖素盆栽防效仅为13%,纳米NANO ZnO和阿泰灵防效分别为3%、15%,防治效果较差。【结论】植物免疫诱抗剂智能聪具有较大的生防潜力,可用于烟草青枯病的绿色防控。【Objective】In order to explore the plant immune inducers which can be used to control tobacco bacterial wilt,the antibacterial activity and control effects of four kinds of inducers were screened and determined.【Method】The control effects were determined and analyzed by using plate inhibition method and potted plant experiments,and the control effects of different plant immune inducers against tobacco bacterial wilt were studied through the determination of minimum inhibition concentration(MIC)and minimum bactericidal concentration(MBC)value.【Result】Aminooligosaccharin and NANO ZnO showed a certain inhibitory effect on Ralstonia solanacearum in the plate tests.The 5000μg/mL amino-oligosaccharin had the best antibacterial effect with the inhibition zone diameter of 35.2 mm,and its MIC and MBC were 1000μg/mL and 2000μg/mL,respectively.Different concentrations of Zhinengcong and Atarin did not show any inhibitory effect.However,the potted plant experiments showed that Zhinengcong had the best prevention and control effect on bacterial wilt.The disease entered the peak period on the 10th day after inoculation,and the control effect reached 57%,which was significantly higher than the 37%control effect of streptomycin treatment in the same period.Moreover,Zhinengcong treatment could delay the occurrence of the disease.The control effect of amino-oligosaccharin with significant antibacterial effect on the plate was only 13%,and the control effects of NANO ZnO and Atarin were 3%and 15%,respectively,with poor control effects.【Conclusion】Plant immune inducer Zhinengcong had a great potential for biocontrol and can be used for green prevention and control of tobacco bacterial wilt.

关 键 词：烟草青枯病 植物免疫诱抗剂 抑菌圈 盆栽防效 生物防治 

分 类 号：S476.8[农业科学—农业昆虫与害虫防治]