冷冻精液室温暴露时间对前向运动精子冷冻复苏率的影响  

Effect of exposure time of frozen semen in room temperature on the freezing recovery rate of forward motile sperm

作　　者：邓顺美[1] 马春杰[1] 庞韬[1] 王奇玲[1] 刘晓华 唐雨倩 李倩仪 朱胜辉 李月华[1] 张欣宗[1] DENG Shunmei;MA Chunjie;PANG Tao;WANG Qiling;LIU Xiaohua;TANG Yuqian;LI Qianyi;ZHU Shenghui;LI Yuehua;ZHANG Xinzong(Guangdong Provincial Family planning Research Institute of Science and Technology,Male Reproductive and Genetic Key Laboratory of the National Health Commission,Guangzhou,Guangdong Province,510600)

机构地区：[1]广东省计划生育科学技术研究所国家卫生健康委员会男性生殖与遗传重点实验室,广州510600

出　　处：《中国计划生育学杂志》2021年第6期1221-1223,共3页Chinese Journal of Family Planning

基　　金：广州市科学研究专项一般项目(编号201607010137)。

摘　　要：目的:研究冷冻精液在室温下反复暴露时间对复苏精液质量的影响。方法:选取2018年广东省计划生育专科医院人类精子库筛查期10例供精者的40份精液标本,按照精液在室温(26℃)下暴露时间长短分为1min组、2min组、4min组、对照组(未暴露于室温),分别检测反复在室温下暴露20次的不同组别的冷冻精液复苏前后的前向运动精子百分率,并计算前向运动精子冷冻复苏率。结果:对照组前向运动精子冷冻复苏率[(73.84±2.53)%]显著高于2min组[(71.15±.21)%]、4min组[(67.65±0.94)%](P<0.05),与1min组[(72.83±0.79)%]无差异(P>0.05);1min组前向运动精子冷冻复苏率显著高于2min组(P<0.05)和4min组(P<0.05);2min组前向运动精子冷冻复苏率显著高于4min组(P<0.05)。结论:冷冻精液在室温下反复暴露≥2min对前向运动精子冷冻复苏率有显著影响,且冷冻精液在室温暴露时间越长,前向运动精子冷冻复苏率越低。冷冻精液在室温下反复暴露≤1min对前向运动精子冷冻复苏率无显著影响。Objective: To study the effect of repeated exposure time in room temperature of frozen semen on the quality of resuscitated semen. Methods: 40 semen samples from 10 donors in the screening period from human sperm bank of Guangdong family planning hospital in 2018 were divided into 4 groups according to the exposure time of semen in room temperature(26 ℃), which included group A(semen exposed in room temperature 1 minute for 20 times), group B(semen exposed in room temperature 2 minutes for 20 times), group C(semen exposed in room temperature 4 minutes for 20 times), and control group(semen without exposed in room temperature). The percentage of forward motile sperm before and after frozen semen recovery in the four groups was detected. The freezing recovery rate of forward motile sperm in the four groups was calculated. Results: The freezing recovery rate of forward motile sperm in control group was significant higher than that in group B and C(P<0.05), but had no significant different from that in group A(P>0.05). The freezing recovery rate of forward motile sperm in group A was significant higher than that in group B and C(P<0.05). The freezing recovery rate of forward motile sperm in group B was significant higher than that in group C(P<0.05). Conclusion: Repeated exposure in room temperature for more than 2 minutes of frozen semen has significant impact on the freezing recovery rate of forward motile sperm. The longer the exposure time in room temperature of frozen semen, the lower freezing recovery rate of forward motile sperm is. Repeated exposure of frozen semen in room temperature for equal to or less than 1 minute has no significant effect on their freezing recovery rate of forward motile sperm.

关 键 词：冷冻精液 室温暴露 前向运动精子冷冻复苏率 

分 类 号：R714.8[医药卫生—妇产科学]