Angiotensin IV upregulates the activity of protein phosphatase 1α in Neura-2A cells  

作　　者：Dan Wang Peng Xue Xiu Lan Chen Zhen Sheng Xie Fu Quan Yang Li Zheng Tao Xu 

机构地区：[1]National Key Laboratory of Biomacromolecules,Institute of Biophysics,Chinese Academy of Sciences,Beijing 100101,China [2]University of Chinese Academy of Science,Beijing 100049,China

出　　处：《Protein & Cell》2013年第7期520-528,共9页蛋白质与细胞（英文版）

基　　金：the Knowledge Innovation Project of the Chinese Academy of Sciences(KSCX1-YW-02);the National Natural Science Foundation of China(Grant No.30801416).

摘　　要：The peptide angiotensin IV(Ang IV)is a derivative of angiotensin II.While insulin regulated amino peptidase(IRAP)has been proposed as a potential receptor for Ang IV,the signalling pathways of Ang IV through IRAP remain elusive.We applied high-resolution mass spectrometry to perform a systemic quantitative phosphoproteome of Neura-2A(N2A)cells treated with and without Ang IV us-ing sta ble-isotope labeling by amino acids in cell culture(SILAC),and identifi ed a reduction in the phosphorylation of a major Ser/Thr protein phosphorylase 1(PP1)upon Ang IV treatment.In addition,spinophilin(spn),a PP1 reg-ulatory protein that plays important functions in the neural system,was expressed at higher levels.Immunoblotting revealed decreased phosphorylation of p70S6 kinase(p70S6K)and the major cell cycle modulator retinoblas-toma protein(pRB).These changes are consistent with an observed decrease in cell proliferation.Taken together,our study suggests that Ang IV functions via regulating the activity of PP1.

关 键 词：angiotensin IV PP1α cell proliferation p70S6 kinase SPINOPHILIN 

分 类 号：R54[医药卫生—心血管疾病]