Origin and molecular characterization of the human-infecting H6N1 infl uenza virus in Taiwan  

作　　者：Weifeng Shi Yi Shi Ying Wu Di Liu George F.Gao 

机构地区：[1]School of Medical Sciences,Taishan Medical College,Taian 271016,China [2]CAS Key Laboratory of Pathogenic Microbiology and Immunology,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [3]Beijing Institutes of Life Sciences,Chinese Academy of Sciences,Beijing 100101,China [4]Network Information Center,Institute of Microbiology,Chinese Academy of Sciences,Beijing 100101,China [5]Offi ce of Director-General,Chinese Center for Disease Control and Prevention(China CDC),Beijing 102206,China

出　　处：《Protein & Cell》2013年第11期846-853,共8页蛋白质与细胞（英文版）

基　　金：the National Basic Research Pro-gram(973 Program)(Nos.2010CB530303,2011CB504703,and 2012CB955501;an intramural special grant for influenza virus research from the Chinese Academy of Sciences(KSZD-EW-Z-002);the Doctoral Starting up Foundation of Taishan Medical College.GFG is a leading principal investigator of the Innova-tive Research Group of the National Natural Science Foundation of China(Grant No.81021003)。

摘　　要：In June 2013,the fi rst human H6N1 infl uenza virus infec-tion was confirmed in Taiwan.However,the origin and molecular characterization of this virus,A/Taiwan/2/2013(H6N1),have not been well studied thus far.In the present report,we performed phylogenetic and coalescent analy-ses of this virus and compared its molecular profi le/char-acteristics with other closely related strains.Molecular characterization of H6N1 revealed that it is a typical avian infl uenza virus of low pathogenicity,which might not rep-licate and propagate well in the upper airway in mammals.Phylogenetic analysis revealed that the virus clusters with A/chicken/Taiwan/A2837/2013(H6N1)in seven genes,except PB1.For the PB1 gene,A/Taiwan/2/2013 was clus-tered with a different H6N1 lineage from A/chicken/Taiwan/A2837/2013.Although a previous study demonstrated that the PB2,PA,and M genes of A/Taiwan/2/2013 might be derived from the H5N2 viruses,coalescent analyses revealed that these H5N2 viruses were derived from more recent strains than that of the ancestor of A/Taiwan/2/2013.Therefore,we propose that A/Taiwan/2/2013 is a reassor-tant from different H6N1 lineages circulating in chickens in Taiwan.Furthermore,compared to avian isolates,a sin-gle P186L(H3 numbering)substitution in the hemaggluti-nin H6 of the human isolate might increase the mammali-an receptor binding and,hence,this strain’s pathogenicity in humans.Overall,human infection with this virus seems an accidental event and is unlikely to cause an infl uenza pandemic.However,its co-circulation and potential reas-sortment with other infl uenza subtypes are still worthy of attention.

关 键 词：molecular characterization phylogenetic analysis coalescent analysis H6N1 infl uenza virus TAIWAN 

分 类 号：S85[农业科学—兽医学]