Pilot study on binding of bovine salivary proteins to grit silicates and plant phytoliths  

作　　者：Marcus MAU Thomas MKAISER Karl-Heinz SÜDEKUM 

机构地区：[1]Saliva Research Unit,Dental Institute,King’s College London,London,UK [2]Zoological Institute and Museum,University of Hamburg,Hamburg,Germany [3]Institute of Animal Science,University of Bonn,Bonn,Germany

出　　处：《Zoological Research》2013年第S03期87-92,共6页动物学研究（英文）

基　　金：This research was supported by the German Research Foundation(DFG,SU 124/15-1).

摘　　要：Mostly fed with grass in fresh or conserved form,cattle and other livestock have to cope with silicate defence bodies from plants(phytoliths)and environmental silicates(grit),which abrade tooth enamel and could additionally interact with various salivary proteins.To detect potential candidates for silicate-binding proteins,bovine whole saliva was incubated with grass-derived phytoliths and silicates.Interactions of salivary proteins with pulverized bovine dental enamel and dentine were additionally analysed.After intense washing,the powder fractions were loaded onto 1D-polyacrylamide gels,most prominent adhesive protein bands were cut out and proteins were identified by mass spectrometry within three independent replicates.All materials were mainly bound by bovine odorant-binding protein,bovine salivary protein 30×10^(3) and carbonic anhydrase VI.The phytolith/silicate fraction showed additional stronger interaction with haemoglobinβand lactoperoxidase.Conceivably,the binding of these proteins to the surfaces may contribute to biological processes occurring on them.

关 键 词：Bovine salivary proteins Mass spectrometry PHYTOLITHS SILICATES Tooth enamel 

分 类 号：S823[农业科学—畜牧学] Q48[农业科学—畜牧兽医]