八珍益智方中非挥发性成分在大鼠体内的代谢产物  

Metabolites of non⁃volatile components from Bazhen Yizhi Prescription in rats in vivo

作　　者：卜凡淑 牛阳[2] 张婷[1] 黄楷迪 钱大玮 段金廒 BU Fan-shu;NIU Yang;ZHANG Ting;HUANG Kai-di;QIAN Da-wei;DUAN Jin-ao(Jiangsu Provincial Collaborative Innovation Center for Industrialization of Chinese Medicinal Resources,Jiangsu Provincial Key Laboratory for High Technology in Research of Chinese Herbal Formulae,Nanjing University of Chinese Medicine,Nanjing 210023,China;Ministry of Education Key Laboratory for Modernization of Hui Medicine,Ningxia Medical University,Yinchuan 750004,China;National and Local Collaborative Engineering Center for Industrialization of Chinese Medicinal Resources and Innovations in Chinese Herbal Formulae,Nanjing University of Chinese Medicine,Nanjing 210023,China)

机构地区：[1]南京中医药大学,江苏省中药资源产业化过程协同创新中心,江苏省方剂高技术研究重点实验室,江苏南京210023 [2]宁夏医科大学回医药现代化教育部重点实验室,宁夏银川750004 [3]中药资源产业化与方剂创新药物国家地方联合工程研究中心,江苏南京210023

出　　处：《中成药》2021年第7期1690-1698,共9页Chinese Traditional Patent Medicine

基　　金：宁夏回族自治区重点研发计划(东西部科技合作)项目(2017BY084)。

摘　　要：目的分析八珍益智方中非挥发性成分在大鼠体内的代谢产物。方法大鼠灌胃给予八珍益智方水煎液(13.95 g/kg)后,采用超高效液相色谱⁃线性离子阱⁃静电轨道阱串联质谱联用(UHPLC⁃LTQ⁃Orbitrap⁃MS)法分析其血浆、胆汁、尿液、粪便中的非挥发性成分。结果共鉴定出6个原型及40个代谢产物,主要发生Ⅰ相代谢反应(氧化、去甲基化等)、II相代谢反应(磺化、葡萄糖醛酸化、甘氨酸或谷胱甘肽结合等)。结论该方法快速有效,可用于鉴定大鼠体内八珍益智方代谢产物,并为进一步研究该复方制剂的功效物质基础和药动学提供依据。AIM To analyze the metabolites of non⁃volatile components from Bazhen Yizhi Prescription in rats in vivo.METHODS Rats were given intragastric administration of water decoction of Bazhen Yizhi Prescription(13.95 g/kg),after which ultra⁃high performance liquid chromatography⁃linear ion trap⁃electrostatic orbit trap tandem mass spectrometry(UHPLC⁃LTQ⁃Orbitrap⁃MS)method was adopted in the analysis of non⁃volatile components in the plasma,bile,urine and fece.RESULTS Total six prototype components and forty metabolites were identified,which mainly occurred PhaseⅠmetabolic reactions(oxidation,demethylation,etc.)and Phase II metabolic reactions(sulfonation,glucuronidation,glycine or glutathione binding,etc.).CONCLUSION This rapid and effective method can be used for the identification of metabolites of Bazhen Yizhi Prescription in rats in vivo,and provide a basis for the further research of effective material basis and pharmacokinetics of this compound preparation.

关 键 词：八珍益智方 非挥发性成分 代谢产物 超高效液相色谱⁃线性离子阱⁃静电轨道阱串联质谱联用(UHPLC⁃LTQ⁃Orbitrap⁃MS) 

分 类 号：R969.1[医药卫生—药理学]