4种苦参生物碱与洗必泰对变形链球菌的联合作用研究  

Combinatorial Effects of Four Alkaloids with Chlorhexidine on Streptococcus mutans

作　　者：杨若琪 赵贵萍 王川东 王静[1] YANG Ruo-qi;ZHAO Gui-ping;WANG Chuan-dong;WANG Jing(College of Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;College of Chinese Medicine,Yunnan University of Chinese Medicine,Kunming 650500,China;Institute of Microbial Technology,Shandong University,Qingdao 266237,China)

机构地区：[1]山东中医药大学药学院,山东济南250355 [2]云南中医药大学中药学院,云南昆明650500 [3]山东大学微生物技术研究院,山东青岛266237

出　　处：《现代中药研究与实践》2021年第3期34-38,共5页Research and Practice on Chinese Medicines

基　　金：山东省中医药科技发展计划项目(2019-0027)。

摘　　要：目的评估苦参中的4种主要生物碱与洗必泰对变形链球菌的浮游细胞代谢活性以及生物被膜形成的协同抑制作用。方法通过MTT法测定了4种生物碱对变形链球菌浮游细胞代谢活性的影响,通过结晶紫染色法测定了4种生物碱对生物被膜形成的抑制作用,通过棋盘法以及零相互作用效能模型(ZIP)分析了4种生物碱与洗必泰对变形链球菌的协同抑制作用。结果4种生物碱单独处理时对变形链球菌浮游细胞的代谢活性以及生物被膜的形成都表现出浓度依赖性的抑制作用,与洗必泰联合使用时也对变形链球菌具有不同程度的协同抑制作用,其中苦参碱与洗必泰的协同活性最强。结论苦参中的4种主要生物碱增强了洗必泰对变形链球菌浮游细胞和生物被膜细胞的体外抗菌活性,为天然药物与传统抗菌药物的联用提供了新思路。Objective To evaluate the synergistic inhibitory effects of the four major alkaloids in Sophora flavescens with chlorhexidine on the planktonic cell metabolism and biofilm formation of Streptococcus mutans.Methods The effects of the four alkaloids on the metabolic activity of S.mutans planktonic cells are determined by MTT method,and the inhibitory effects of the four alkaloids on the biofilm formation are determined by crystal violet staining.The checkerboard method and Zero Interaction Potency model(ZIP)analyzed the synergistic inhibitory effects of the four alkaloids with chlorhexidine on S.mutans.Results The four alkaloids exhibited a does-dependent inhibitory effect on the metabolic activity of plankton cells and the biofilm formation of S.mutans after individual treatment.When used in combination with chlorhexidine,they also has different degrees of synergistic inhibitory effects on S.mutans.Among them,matrine have the strongest synergistic activity with chlorhexidine.Conclusion The four alkaloids in Sophora flavescens enhance the antibacterial activity of chlorhexidine against S.mutans planktonic cells and biofilm cells,providing new light on the potential to develop natural medicine in combination with traditional antibacterial agents as treatments to arrest dental caries.

关 键 词：苦参 生物碱 洗必泰 变形链球菌 生物被膜 

分 类 号：R285.5[医药卫生—中药学]