动脉旁路移植术后静脉桥再狭窄通路的机制  

Signal Pathway Related Mechanism of Vein Graft Failure in Coronary Artery Bypass Grafting

作　　者：张浒[1] 彭安睿 钟佳冀 黄波[2] ZHANG Hu;PENG An-rui;ZHONG Jia-ji;HUANG Bo(Dept.of Cardiavascular Surgery,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China;Dept.Of Operating Room,The 1st Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650032,China)

机构地区：[1]昆明医科大学第一附属医院心脏大血管外科,云南昆明650032 [2]昆明医科大学第一附属医院麻醉手术科,云南昆明650032

出　　处：《昆明医科大学学报》2021年第7期20-24,共5页Journal of Kunming Medical University

基　　金：云南省科技厅-昆明医科大学应用基础研究联合专项基金资助项目[2017FE467(-029)];云南省教育厅科学研究基金资助项目(2021J0242)。

摘　　要：目的探索动脉旁路移植术后静脉桥再狭窄形成的信号通路机制。方法40只家兔随机分2组,假手术组和模型组,假手术组分离颈静脉、颈动脉,不做移植,模型组游离颈静脉,截取一段颈静脉方向调转后与颈动脉做端侧吻合,结扎两吻合口间的颈动脉,建立动脉旁路移植兔模型,移植后2 d、2周假手术组取出颈静脉,模型组取出移植静脉,肉眼观察,组织切片HE染色量化管腔狭窄程度,免疫组化检测p-p38表达水平,进行对比分析。结果与假手术组比较,模型组移植静脉2周后管腔显著性狭窄(P<0.05),静脉移植后2 d、2周p-p38表达均显著性升高(P<0.05),其中移植后2 d显著高于移植2周(P<0.05)。结论冠状动脉旁路移植静脉桥再狭窄信号传导机制可能通过p38-MAPK通路。Objective To explore the signal pathway of vein graft failure in coronary artery bypass grafting.Methods 40 rabbits were randomly divided into two groups.Group A was sham operation group;Group B was model group in which rabbit model of CABG was established.The jugular vein in group A and the graft vein group B was removed 2 days and 2 weeks after the vein grafting,and pathological section was used to quantify lumen stenosis,immunohistochemistry was used to detect the phosphorylated p38,and the indexes were comared.Results Compared with group A,the degree of luminal stenosis of group B were significantly higher in 2 weeks after vein graft(P<0.05).The expression of p-p38 in vein graft tissue of group B were significantly higher than group A in 2 days and 2 weeks after vein grafting,p-p38 were significantly higher 2 days than 2 weeks after vein grafting in group B(P<0.05).Conclusion The mechanism of vein graft failure in CABG might be related to the p38 MAPK signal pathway.

关 键 词：冠状动脉旁路移植术 静脉桥再狭窄 信号通路 p38 MAPK 兔 

分 类 号：R332[医药卫生—人体生理学]