三色片醇提物抗补体活性物质的UPLC-Q-TOF-MS/MS研究  

Study on components with anti-complement activity from ethanol extract of Sanse tablets by UPLC-Q-TOF-MS/MS

作　　者：秦艳 陶佳佳 沈赟 陈喆 叶岩荣 QIN Yan;TAO Jiajia;SHEN Yun;CHEN Zhe;YE Yanrong(Department of Pharmacy,Zhongshan Hospital,Fudan University,Shanghai 200032)

机构地区：[1]复旦大学附属中山医院药剂科,上海200032

出　　处：《药学实践杂志》2021年第4期322-330,共9页Journal of Pharmaceutical Practice

基　　金：复旦大学附属中山医院青年基金项目(2018ZSQN27)。

摘　　要：目的分析三色片醇提物抗补体活性部分的化学成分,为阐明其药效物质基础提供参考依据。方法通过经典抗补体途径对不同极性部位的三色片醇提物进行活性测定,确定其具有抗补体活性的部位,采用超高效液相色谱-电喷雾电离-四级杆-飞行时间质谱联用(UPLC-ESI-Q-TOF-MS/MS)法对活性部位进行成分分析。通过与对照品的保留时间,精确分子量和参考文献中质谱裂解碎片比对,对其中的化学成分进行鉴定。结果三色片醇提物的乙酸乙酯部位具有较强的抗补体活性,应用质谱分析,鉴定出42个化合物,化合物的类型包括:生物碱类16个,萜类15个,黄酮6个和酚酸类5个。结论通过对三色片中抗补体活性部位化学成分特征的分析,为阐明其药效物质基础提供一定的科学依据,有助于院内中药复方制剂的二次开发。Objective To analyze the chemical components in the ethanol extract of Sanse tablets with anti-complement activity,and to provide scientific basis for its therapeutic effects.Methods The classical anti-complement pathway was used to compare the activity of different portions of Sanse tablet alcohol extract,and to identify the fraction with anti-complement activity.The chemical composition in active fraction was analyzed by UPLC-Q-TOF-MS/MS.The chemical components were identified by comparing the retention time,exact molecular weight and mass spectrum information with the standard chemicals.Results The ethyl acetate fraction of the Sanse tablet ethanol extract had the best anti-complement activity.42 chemicals were identified,including 16 alkaloids,15 terpenoids,6 flavonoids and 5 phenolic acids.Conclusion The characterization of the chemical components in the anti-complement active fraction of Sanse tablets provides a scientific basis for the therapeutic effects of Sanse tablets,which will help the future development of the compound preparations of Chinese medicine in our hospital.

关 键 词：三色片 抗补体活性 UPLC-Q-TOF-MS/MS 化学成分 

分 类 号：R284.1[医药卫生—中药学]