塔宾曲霉L-27菌株代谢产物柠檬酸及其酯类的分离与抑制植物幼苗生长活性  

Isolation of Citric Acid and Its Esters from Aspergillus tubingensis L-27 Strain and Their Activities against Plant Seedling Growth

作　　者：宋玉婕 杨从军 SONG Yu-jie;YANG Cong-jun(Key Lab of Integrated Crop Pest Management of Shandong Province,College of Plant Health and Medicine,Qingdao Agricultural University,Qingdao 266109,Shandong,China)

机构地区：[1]青岛农业大学植物医学学院山东省农作物病虫害综合防控重点实验室,山东青岛266109

出　　处：《农药》2021年第7期489-492,共4页Agrochemicals

基　　金：山东省重点研发计划(2019GNC106150)。

摘　　要：[目的]分离塔宾曲霉L-27菌株发酵液中抑制植物幼苗生长的活性成分。[方法]采用弱碱性阴离子树脂D301富集、0.1 mol/L HCl乙醇溶液解吸、有机溶剂萃取、硅胶柱色谱分离,1H NMR和13C NMR鉴定。[结果]追踪分离得到对称二乙酯柠檬酸(1)、柠檬酸-1-乙酯(2)、柠檬酸(3)。在1250 mg/L,3个化合物对小麦幼苗根(茎)抑制率分别为100.0%(67.5%)、100.0%(59.7%)、100.0%(55.7%)。利用化合物3标准品测定生物活性,对小麦幼苗根(茎)抑制的EC50值为465.1 mg/L(1043.4 mg/L);对反枝苋和马齿苋幼苗全株高的抑制EC50值分别为486.9、652.2 mg/L。[结论]柠檬酸、柠檬酸-1-乙酯和对称二乙酯柠檬酸具有衍化开发除草剂的潜力。[Aims] This study aims to isolate active components for inhibition of plant seedling growth in the fermentation broth of Aspergillus tubingensis L-27 strain. [Methods] Weak basic anion resin D301 enrichment, 0.1 mol/L HCl ethanol solution desorption, organic solvent extraction, silica gel column chromatography,1H NMR and 13C NMR were adopted. [Results] Symmetric diethyl ester citric acid(1), citric acid-1-ethyl ester(2) and citric acid(3) were isolated.At 1250 mg/L, the inhibition rates of three compounds on root(stem) of wheat seedlings were 100.0%(67.5%), 100.0%(59.7%) and 100.0%(55.7%), respectively. Using compound 3 standard to determine toxicity, EC50 of inhibiting wheat seedling root(stem) was 465.1(1043.4) mg/L, and EC50 of inhibiting whole plant height on Amaranthus retroflexus and Portulaca oleracea were 486.9 and 652.2 mg/L, respectively. [Conclusions] Isolated symmetric diethyl ester citric acid,citric acid-1-ethyl ester and citric acid had potential to be derived for development of herbicides.

关 键 词：塔宾曲霉L-27菌株 对称二乙酯柠檬酸 柠檬酸-1-乙酯 柠檬酸 植物幼苗生长抑制 

分 类 号：S482.4[农业科学—农药学]