纤枝短月藓LEA5基因表达及耐盐性分析《西北植物学报》发表论文的院士作者统计  

Expression and Salt Tolerance of LEA5 Gene from Brachymenium exile

作　　者：李雪宝 周璇 鄢波[1] LI Xuebao;ZHOU Xuan;YAN Bo(College of Landscape Architecture and Horticulture Sciences, Southwest Forestry University, Kunming 650224, China)

机构地区：[1]西南林业大学园林园艺学院,昆明650224

出　　处：《西北植物学报》2021年第6期919-925,共7页Acta Botanica Boreali-Occidentalia Sinica

基　　金：国家自然科学基金(31160177);国家林业局西南风景园林工程技术研究中心;云南省功能性花卉资源及产业化技术工程研究中心;云南省面向南亚东南亚经济林全产业链联合研发中心。

摘　　要：该研究以纤枝短月藓为材料,利用RT-PCR和HiTail-PCR技术分别克隆得到纤枝短月藓LEA5基因的ORF和启动子序列,并进行生物信息学、基因表达及耐盐性分析,为进一步研究LEA5蛋白的保护机制奠定基础。结果显示:(1)LEA5基因包含267 bp的开放阅读框(ORF),编码88个氨基酸。(2)LEA5基因启动子序列为1053 bp,利用PlantCARE在线工具预测顺式作用元件显示,该启动子不仅具有典型的CAAT box元件,还含有ABRE、MYB、MYC、MYB结合位点(MBS)等其他元件。(3)荧光定量分析表明,LEA5基因在纤枝短月藓不同时期和不同组织中都有表达。(4)LEA5蛋白的异源表达提高了大肠杆菌对盐胁迫的耐受性,表明LEA5蛋白可能在耐盐性中起重要作用。LEA5 gene was isolated from Brachymenium exile by polymerase chain reaction(RT-PCR)and the promoter sequence of LEA5 gene was isolated by High-efficiency thermal asymmetric interlaced polymerase chain reaction(HiTail-PCR).The bioinformatics,expression characteristics and salt stress tolerance of LEA5 were analyzed.This research laid a foundation for further investigating the protective mechanisms of LEA5 proteins.The results showed that:(1)LEA5 gene contains 267 bp open reading frame(ORF),which encodes 88 amino acids.(2)The promoter sequence of the 1053 bp LEA5 gene was analyzed by PlantCARE showed that it had CAAT box,ABRE,MYB,MYC,MYB binding site and other cis-acting elements.(3)Quantitative real-time PCR analysis indicated that LEA5 gene expressed in different stages and tissues of B.exile.(4)In addition,the heterologous expression of LEA5 protein increased the tolerance of Escherichia coli to salt stress.These results suggested that LEA5 protein might have an important function in the tolerance of the salt stress.

关 键 词：纤枝短月藓 LEA5基因 启动子 盐胁迫 表达 

分 类 号：Q785[生物学—分子生物学] Q786